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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Overview
Product name
Anti-PKA 2 beta (regulatory subunit) antibody
See all PKA 2 beta (regulatory subunit) products (8) ...
Description
Rabbit polyclonal to PKA 2 beta (regulatory subunit)
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A KLH conjugated synthetic peptide selected from the sequence of human PKA 2 beta within aa 1-100, (N terminal region).
Positive control
Mouse brain tissue lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Western blot - PKA 2 beta (regulatory subunit) antibody (ab38222)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Relevance
PKA 2 beta (regulatory subunit or PRKAR2B) is one of the regulatory subunits of cAMP-dependent Protein Kinase (PKA), which is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits (R1 alpha, R1 beta, R2 alpha, and R2 beta). Upon adenylate cyclase activation, AMP binds to the regulatory subunits, and releases the catalytic subunit, which becomes active upon its dissociation from the regulatory subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
Cellular localization
Cytoplasmic
Alternative names
- AI451071 antibody
- AW061005 antibody
- cAMP dependent protein kinase type II beta regulatory chain antibody
see all
Database links
- Entrez Gene: 5577 Human
- Entrez Gene: 19088 Mouse
- Omim: 176912 Human
- SwissProt: P31323 Human
- SwissProt: Q6DHZ2 Human
- SwissProt: P31324 Mouse
- Unigene: 433068 Human
Anti-PKA 2 beta (regulatory subunit) antibody images:
Western blot - PKA 2 beta (regulatory subunit) antibody (ab38222)
Anti-PKA 2 beta (regulatory subunit) antibody (ab38222) at 1/100 dilution + mouse brain tissue lysate
Predicted band size : 46 kDa
Observed band size : 46 kDa
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
References for Anti-PKA 2 beta (regulatory subunit) antibody (ab38222)
ab38222 has not yet been referenced specifically in any publications.
Publishing research using ab38222? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"