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Western blot - PKA 2 beta (regulatory subunit) antibody (ab38222)
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Product Name
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PKA 2 beta (regulatory subunit) antibody
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See all PKA 2 beta (regulatory subunit) antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PKA 2 beta (regulatory subunit)
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Immunogen
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A KLH conjugated synthetic peptide selected from the sequence of human PKA 2 beta within aa 1-100, (N terminal region).
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse brain tissue lysate
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Relevance
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PKA 2 beta (regulatory subunit or PRKAR2B) is one of the regulatory subunits of cAMP-dependent Protein Kinase (PKA), which is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits (R1 alpha, R1 beta, R2 alpha, and R2 beta). Upon adenylate cyclase activation, AMP binds to the regulatory subunits, and releases the catalytic subunit, which becomes active upon its dissociation from the regulatory subunit.
This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PKA 2 beta (regulatory subunit) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PKA 2 beta (regulatory subunit) antibody images, references, products related to ab38222 and other tools.
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PKA 2 beta (regulatory subunit) antibody images:
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 Western blot - PKA 2 beta (regulatory subunit) antibody (ab38222)
PKA 2 beta (regulatory subunit) antibody (ab38222) at 1/100 dilution + mouse brain tissue lysate
Predicted band size : 46 kDa
Observed band size : 46 kDa
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Search PubMed (MEDLINE) for references to PKA 2 beta (regulatory subunit)
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PKA 2 beta (regulatory subunit) antibody - more information
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