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- PKA 2 beta (regulatory subunit) (phospho S114) antibody (ab5829)
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Product Name
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PKA 2 beta (regulatory subunit) (phospho S114) antibody
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See all PKA 2 beta (regulatory subunit) antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PKA 2 beta (regulatory subunit) (phospho S114)
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Immunogen
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Synthetic phosphopeptide derived from a region of human PKA regulatory 2 beta subunit that contains serine 114.
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Cow, Rb
(Due to sequence homology)
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Specificity
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PKA regulatory 2 alpha subunit (75% homologous) has not been tested.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 50 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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3T3-L1 adipocytes or Y1 mouse adrenal cortical cells.
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Relevance
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PKA 2 beta (regulatory subunit or PRKAR2B) is one of the regulatory subunits of cAMP-dependent Protein Kinase (PKA), which is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits (R1 alpha, R1 beta, R2 alpha, and R2 beta). Upon adenylate cyclase activation, AMP binds to the regulatory subunits, and releases the catalytic subunit, which becomes active upon its dissociation from the regulatory subunit.
This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKA. The final product is generated by
affinity chromatography using a PKA-derived peptide that is phosphorylated at serine 114.
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PKA 2 beta (regulatory subunit) (phospho S114) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PKA 2 beta (regulatory subunit) (phospho S114) antibody images, references, products related to ab5829 and other tools.
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PKA 2 beta (regulatory subunit) (phospho S114) antibody images:
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 - PKA 2 beta (regulatory subunit) (phospho S114) antibody (ab5829)
Peptide Competition and Phosphatase Treatment: Lysates prepared from 3T3-L1 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, and incubated with ab5829 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA reg 2 beta [pS114] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
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Search PubMed (MEDLINE) for references to PKA 2 beta (regulatory subunit)
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PKA 2 beta (regulatory subunit) (phospho S114) antibody - more information
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