You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-PKA R2 antibody [Y116] (ab32514)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-PKA R2 antibody [Y116]
See all PKA R2 products (13) ...

Description

Rabbit monoclonal [Y116] to PKA R2

Specificity

The antibody recognises PKA RII. It does not cross-react with other cAMP-dependent kinase.

Tested applications

WB, IHC-P, ICC/IF, IPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in the C-terminus of human cAMP-dependent protein kinase type II-alpha regulatory subunit.

Positive control

K562 cell lysate, HeLa cells and normal breast tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Y116

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cAMP
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA

  • Western blot - PKA R2 antibody [Y116] (ab32514)Western blot - PKA R2 antibody [Y116] (ab32514) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - PKA R2 antibody [Y116] (ab32514)Immunohistochemistry (Paraffin-embedded sections) - PKA R2 antibody [Y116] (ab32514) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - PKA R2 antibody [Y116] (ab32514)Immunocytochemistry/ Immunofluorescence - PKA R2 antibody [Y116] (ab32514) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Tissue specificity

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Sequence similarities

Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.

Post-translational
modifications

Phosphorylated by the activated catalytic chain.

Target information above from: UniProt accessionP13861 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • cAMP dependent protein kinase regulatory subunit alpha 2 antibody
  • cAMP dependent protein kinase regulatory subunit RII alpha antibody
  • cAMP dependent protein kinase type II alpha regulatory chain antibody
  • cAMP dependent protein kinase type II alpha regulatory subunit antibody
  • cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
  • KAP2 antibody
  • KAP2_HUMAN antibody
  • MGC3606 antibody
  • PKR 2 antibody
  • PKR2 antibody
  • PRKA R2 antibody
  • PRKAR 2 antibody
  • PRKAR2 antibody
  • PRKAR2A antibody
  • Protein kinase A RII alpha subunit antibody
  • Protein kinase cAMP dependent regulatory type II alpha antibody
see all

Database links

Anti-PKA R2 antibody [Y116] images:

  Western blot - PKA R2 antibody [Y116] (ab32514)

Western blot - PKA R2 antibody [Y116] (ab32514)

Anti-PKA R2 antibody [Y116] (ab32514) at 1/50000 dilution + K562 cell lysate

Predicted band size : 45 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - PKA R2 antibody [Y116] (ab32514)

Immunohistochemistry (Paraffin-embedded sections) - PKA R2 antibody [Y116] (ab32514)

Immunohistochemical analysis of paraffin-embedded human normal breast tissue using ab32514 at 1/100 dilution.

  Immunocytochemistry/ Immunofluorescence - PKA R2 antibody [Y116] (ab32514)

Immunocytochemistry/ Immunofluorescence - PKA R2 antibody [Y116] (ab32514)

Immunofluorescent staining of HeLa cells using ab32514 at 1/100 dilution.

References for Anti-PKA R2 antibody [Y116] (ab32514)

This product has been referenced in:

  • Gu L  et al. Protein kinase A activation confers resistance to trastuzumab in human breast cancer cell lines. Clin Cancer Res 15:7196-206 (2009). IHC-P; Human.Read more (PubMed: 19920112) »

See 1 publication for this product

Publishing research using ab32514? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"