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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-PKA regulatory subunit I beta antibody
See all PKA regulatory subunit I beta products (5) ...
Description
Rabbit polyclonal to PKA regulatory subunit I beta
Specificity
ab28544 binds to PKARIb, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIIb).
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Human
Immunogen
Synthetic peptide, corresponding to amino acids 60-90 of Rat PKA regulatory subunit I beta(Peptide available as ab41296.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 43 kDa.
This antibody identifies bands at 55 kD and 53 kD when used against the reduced protein as well as activation/breakdown products. The antibody can recognize native or reduced PKARIb. Dilution optimised using Chromogenic detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Tissue specificity
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Sequence similarities
Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
Post-translational
modifications
The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
Target information above from: UniProt accessionP31321
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cAMP dependent protein kinase type I beta regulatory subunit antibody
- cAMP-dependent protein kinase type I-beta regulatory subunit antibody
- KAP1_HUMAN antibody
see all
Database links
- Entrez Gene: 5575 Human
- Entrez Gene: 19085 Mouse
- Entrez Gene: 25521 Rat
- Omim: 176911 Human
- SwissProt: P31321 Human
- SwissProt: Q6NS41 Human
- SwissProt: Q8N422 Human
- SwissProt: P12849 Mouse
see all
Protocols
References for Anti-PKA regulatory subunit I beta antibody (ab28544)
ab28544 has not yet been referenced specifically in any publications.
Publishing research using ab28544? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"