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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Overview
Product name
Anti-PKA regulatory subunit I beta antibody
See all PKA regulatory subunit I beta products (5) ...
Description
Rabbit polyclonal to PKA regulatory subunit I beta
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized peptide derived from an internal region of human PKA regulatory subunit I beta
Positive control
Extracts from COLO205 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Western blot - PKA regulatory subunit I beta antibody (ab70028)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab70028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Tissue specificity
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Sequence similarities
Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
Post-translational
modifications
The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
Target information above from: UniProt accessionP31321
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cAMP dependent protein kinase type I beta regulatory subunit antibody
- cAMP-dependent protein kinase type I-beta regulatory subunit antibody
- KAP1_HUMAN antibody
see all
Database links
- Entrez Gene: 5575 Human
- Entrez Gene: 19085 Mouse
- Entrez Gene: 25521 Rat
- Omim: 176911 Human
- SwissProt: P31321 Human
- SwissProt: Q6NS41 Human
- SwissProt: Q8N422 Human
- SwissProt: P12849 Mouse
see all
Anti-PKA regulatory subunit I beta antibody images:
Western blot - PKA regulatory subunit I beta antibody (ab70028)
All lanes : Anti-PKA regulatory subunit I beta antibody (ab70028) at 1/500 dilution
Lane 1 : extracts from COLO205 cells
Lane 2 : extracts from COLO205 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 43 kDa
Observed band size : 43 kDa
References for Anti-PKA regulatory subunit I beta antibody (ab70028)
ab70028 has not yet been referenced specifically in any publications.
Publishing research using ab70028? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"