Anti-PKC alpha antibody [Y143] (ab32122)

  Western blot - PKC alpha antibody [Y143] (ab32122)

Anti-PKC alpha antibody [Y143] (ab32122) at 1/2000 dilution + K562 cell lysate

Predicted band size : 77 kDa
Observed band size : 77 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

  Immunohistochemistry (Paraffin-embedded sections) - PKC alpha antibody [Y143] (ab32122)

ab32122, staining human carcinoma of thyroid gland by immunohistochemistry, Paraffin embedded tissue

  Immunofluorescence - PKC alpha antibody [Y143] (ab32122)

ab32122, staining of Hela cells by immunofluorescent

  Flow Cytometry - Anti-PKC alpha antibody [Y143] (ab32122)

Overlay histogram showing HeLa cells stained with ab32122 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32122, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.