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Overview

  • Product nameAnti-PKC alpha + beta 2 antibodySee all PKC alpha + beta 2 primary antibodies ...
  • Description
    Rabbit polyclonal to PKC alpha + beta 2
  • SpecificityDetects endogenous levels of total PKC alpha/beta 2 protein.
  • Tested applicationsELISA, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non phosphopeptide derived from human PKC alpha/beta 2 around the phosphorylation site of threonine 638 (VLTPPP).

  • Positive controlHuman breast carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab59328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/5000.
IHC-P IHC-P: Use at an assay dependent dilution.

Target

  • RelevanceProtein kinase C alpha and protein kinase C beta belong to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily. Both contain 1 AGC kinase C terminal domain, 1 C2 domain, 2 phorbol ester/DAG type zinc fingers and 1 protein kinase domain. They are calcium activated, phospholipid dependent, serine and threonine specific enzymes. PKC alpha and may play a role in cell motility. PKC beta may be a novel component of the NF kappa B signaling axis responsible for the survival and activation of B cells after BCR cross linking. Both are activated by diacylglycerol which in turn phosphorylates a range of cellular proteins and both may also serve as the receptor for phorbol esters, a class of tumor promoters. PKC beta has two named isoforms produced by alternative splicing.
  • Cellular localizationCell Membrane and Cytoplasmic
  • Database links
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  • Alternative names
      PKC alpha antibodyPKC beta antibodyPKCA antibody
      PKCB antibodyPRKACA antibodyPRKCB antibodyPRKCB2 antibodyProtein kinase C alpha type antibodyProtein kinase C beta antibodyProtein kinase C beta type antibodyprotein kinase C, alpha antibody
    see all

Anti-PKC alpha + beta 2 antibody images

  • Immunohistochemistry (Paraffin-embedded sections) - PKC alpha + beta 2 antibody (ab59328)
    Immunohistochemistry (Paraffin-embedded sections) - PKC alpha + beta 2 antibody (ab59328)
    ab59328 at 1/50 dilution staining human breast carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.

References for Anti-PKC alpha + beta 2 antibody (ab59328)

ab59328 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"