Anti-PKC alpha + beta 2 (phospho T638) antibody (ab59365)

Overview

  • Product name
    Anti-PKC alpha + beta 2 (phospho T638) antibody
    See all PKC alpha + beta 2 primary antibodies
  • Description
    Rabbit polyclonal to PKC alpha + beta 2 (phospho T638)
  • Specificity
    ab59365 detects endogenous levels of PKC alpha + beta 2 only when phosphorylated at threonine 638.
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized phosphopeptide derived from human PKC alpha + beta 2 around the phosphorylation site of threonine 638 (VLTPPP).

  • Positive control
    • Human breast carcinoma tissue
  • General notes
    Store at -20°C for 1 year

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Protein kinase C alpha and protein kinase C beta belong to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily. Both contain 1 AGC kinase C terminal domain, 1 C2 domain, 2 phorbol ester/DAG type zinc fingers and 1 protein kinase domain. They are calcium activated, phospholipid dependent, serine and threonine specific enzymes. PKC alpha and may play a role in cell motility. PKC beta may be a novel component of the NF kappa B signaling axis responsible for the survival and activation of B cells after BCR cross linking. Both are activated by diacylglycerol which in turn phosphorylates a range of cellular proteins and both may also serve as the receptor for phorbol esters, a class of tumor promoters. PKC beta has two named isoforms produced by alternative splicing.
  • Cellular localization
    Cell Membrane and Cytoplasmic
  • Database links
  • Alternative names
    • PKC alpha antibody
    • PKC beta antibody
    • PKCA antibody
    • PKCB antibody
    • PRKACA antibody
    • PRKCB antibody
    • PRKCB2 antibody
    • Protein kinase C alpha type antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    • protein kinase C, alpha antibody
    see all

Anti-PKC alpha + beta 2 (phospho T638) antibody images

  • ab59365, at a 1/50 dilution, staining human PKC alpha + beta 2 in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).

References for Anti-PKC alpha + beta 2 (phospho T638) antibody (ab59365)

ab59365 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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