Anti-PKC alpha + beta 2 (phospho T638) antibody (ab59365)

Overview

• Product nameAnti-PKC alpha + beta 2 (phospho T638) antibodySee all PKC alpha + beta 2 primary antibodies ...
• Description
  Rabbit polyclonal to PKC alpha + beta 2 (phospho T638)
• Specificityab59365 detects endogenous levels of PKC alpha + beta 2 only when phosphorylated at threonine 638.
• Tested applicationsELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthesized phosphopeptide derived from human PKC alpha + beta 2 around the phosphorylation site of threonine 638 (VLT^PPP).

• Positive controlHuman breast carcinoma tissue
• General notesStore at -20°C for 1 year

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg⁺² and Ca⁺²), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKC

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

Applications

Target

• RelevanceProtein kinase C alpha and protein kinase C beta belong to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily. Both contain 1 AGC kinase C terminal domain, 1 C2 domain, 2 phorbol ester/DAG type zinc fingers and 1 protein kinase domain. They are calcium activated, phospholipid dependent, serine and threonine specific enzymes. PKC alpha and may play a role in cell motility. PKC beta may be a novel component of the NF kappa B signaling axis responsible for the survival and activation of B cells after BCR cross linking. Both are activated by diacylglycerol which in turn phosphorylates a range of cellular proteins and both may also serve as the receptor for phorbol esters, a class of tumor promoters. PKC beta has two named isoforms produced by alternative splicing.
• Cellular localizationCell Membrane and Cytoplasmic
• Database links
  • Entrez Gene: 5578 Human
  • Entrez Gene: 5579 Human
  • Entrez Gene: 18750 Mouse
  • Entrez Gene: 18751 Mouse
  • Entrez Gene: 24680 Rat
  • SwissProt: P17252 Human
  • SwissProt: P05771 Human
  • SwissProt: P20444 Mouse

  • SwissProt: P68404 Mouse
  • SwissProt: P05696 Rat
  • SwissProt: P68403 Rat
  • Unigene: 531704 Human
  • Unigene: 680598 Human
  • Unigene: 207908 Rat

  see all

• Alternative names
  PKC alpha antibodyPKC beta antibodyPKCA antibody

  PKCB antibodyPRKACA antibodyPRKCB antibodyPRKCB2 antibodyProtein kinase C alpha type antibodyProtein kinase C beta antibodyProtein kinase C beta type antibodyprotein kinase C, alpha antibody

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Anti-PKC alpha + beta 2 (phospho T638) antibody images

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  Immunohistochemistry (Paraffin-embedded sections) - PKC alpha + beta 2 (phospho T638) antibody (ab59365)
  ab59365, at a 1/50 dilution, staining human PKC alpha + beta 2 in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).