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Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

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Overview

Product name

Anti-PKC alpha (phospho T638) antibody [E195]
See all PKC alpha products (18) ...

Description

Rabbit monoclonal [E195] to PKC alpha (phospho T638)

Specificity

The antibody only detects PKC alpha phosphorylated on Threonine 638.

Tested applications

WB, IHC-P, ICC/IF, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Chicken, Cow

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

Positive control

293 cell lysate and normal brain tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

E195

Isotype

IgG

Research areas

Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC

  • Western blot - PKC alpha (phospho T638) antibody [E195] (ab32502)Western blot - PKC alpha (phospho T638) antibody [E195] (ab32502) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502)Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502)Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/500.
IHC-P: 1/250.
IP: 1/40.
WB: 1/20000. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.

Cellular localization

Cytoplasm. Cell membrane. Nucleus.

Target information above from: UniProt accessionP17252 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • PKC alpha antibody
  • AAG6 antibody
  • Aging associated gene 6 antibody
  • KPCA_HUMAN antibody
  • MGC129900 antibody
  • MGC129901 antibody
  • PICK 1 antibody
  • PKC A antibody
  • PKC alpha antibody
  • PKC-A antibody
  • PKC-alpha antibody
  • PKCA antibody
  • PRKACA antibody
  • PRKC A antibody
  • PRKCA antibody
  • Protein Kinase C alpha antibody
  • Protein kinase C alpha type antibody
see all

Database links

see all

Anti-PKC alpha (phospho T638) antibody [E195] images:

  Western blot - PKC alpha (phospho T638) antibody [E195] (ab32502)

Western blot - PKC alpha (phospho T638) antibody [E195] (ab32502)

All lanes : Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) at 1/20000 dilution

Lane 1 : 293 cell lysate, untreated
Lane 2 : 293 cell lysate, treated with TPA.


Predicted band size : 77 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502)

Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502)

Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution.

  Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502)

Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502)

Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution

References for Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

ab32502 has not yet been referenced specifically in any publications.

Publishing research using ab32502? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"