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Purified bovine brain protein kinase C
Use: It should be possible to selectively block degradation of PKC without affecting the membrane associated activation. This will allow an assessment of the role of proteolysis in the activation of the protein kinase pathway.
Our Abpromise guarantee covers the use of ab31 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18368118|
|IHC-P||Use at an assay dependent concentration. PubMed: 17266784|
Vero cells with ab31 at a 1/50 dilution. This picture was kindly submitted as part of the review by Martha Simpson-Holley.
WB using ab31. Lane 1. Calpain treated PKC from Sigma . Lane 2. Homogenate from rat adipocytes. Lane 3. Homogenate from 3T3-L1 cells (mouse). Lane 4. Purified microsomes from 3T3-L1 cells. Review by Mikael Rutberg submitted 2 September 2004.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"