Overview

  • Product nameAnti-PKC antibodySee all PKC primary antibodies ...
  • Description
    Rabbit polyclonal to PKC
  • Specificityab19031 recognizes the alpha, beta and gamma isoforms of PKC.
  • Tested applicationsICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to the C terminus of Human PKC

Properties

Applications

Our Abpromise guarantee covers the use of ab19031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 82 kDa.

Target

Anti-PKC antibody images

  • Anti-PKC antibody (ab19031) at 0.5 µg/ml + Cell lysate prepared from rat kidney cells

    Predicted band size : 82 kDa
  • ICC/IF image of ab19031 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19031, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PKC antibody (ab19031)

ab19031 has not yet been referenced specifically in any publications.

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