Overview

  • Product nameAnti-PKC antibody
    See all PKC primary antibodies
  • Description
    Rabbit polyclonal to PKC
  • Specificityab59363 detects endogenous levels of total PKC protein.
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized non-phosphopeptide derived from human PKC around the phosphorylation site of threonine 497 (T-S-TP-F-C).

  • Positive control
    • NIH/3T3 cytoplasmic or membrane lysate Human breast carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography, using epitope-specific immunogen.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab59363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 81 kDa.
IHC-P 1/50 - 1/100.

Target

Anti-PKC antibody images

  • All lanes : Anti-PKC antibody (ab59363) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins), minus immunising peptide
    Lane 2 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins), plus immunising peptide


    Observed band size : 81 kDa (why is the actual band size different from the predicted?)
  • ab59363, at a 1/50 dilution, staining Human PKC in breast carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).

References for Anti-PKC antibody (ab59363)

This product has been referenced in:
  • Leiria LO  et al. Role of PKC and CaV1.2 in detrusor overactivity in a model of obesity associated with insulin resistance in mice. PLoS One 7:e48507 (2012). WB ; Mouse . Read more (PubMed: 23144896) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"