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Anti-PKC beta 1 + PKC beta 2 antibody (ab5627)

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Overview

  • Product nameAnti-PKC beta 1 + PKC beta 2 antibodySee all PKC beta 1 + PKC beta 2 primary antibodies ...
  • Description
    Rabbit polyclonal to PKC beta 1 + PKC beta 2
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide: SEFEGFSFVNSE, corresponding to amino acids 654-665 of Human Protein kinase C beta II, this sequence is 100% conserved in bovine, rat and mouse PKC beta I and pig, human, rabbit and rat PKC beta II.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab5627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).
    This antibody has been tested against the recombinant human protein only.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications      Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
    • Cellular localizationCytoplasm. Nucleus. Membrane.

      • Target information above from: UniProt accession P05771 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        KPCB_HUMAN antibodyMGC41878 antibodyPKC B2 antibody
        PKC beta antibodyPKC beta II antibodyPKC-B antibodyPKC-beta antibodyPKCB antibodyPKCB2 antibodyPKCB2 antibodyPKCBII antibodyPRKC B2 antibodyPrkcb antibodyPRKCB I antibodyPRKCB II antibodyPRKCB1 antibodyPRKCB2 antibodyProtein kinase C beta 1 antibodyProtein kinase C beta 1 polypeptide antibodyProtein kinase C beta 1 polypeptide antibodyProtein kinase C beta 2 antibodyProtein kinase C beta 2 antibodyProtein kinase C beta antibodyProtein kinase C beta type antibody
      see all

    References for Anti-PKC beta 1 + PKC beta 2 antibody (ab5627)

    ab5627 has not yet been referenced specifically in any publications.

    Publishing research using ab5627 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab5627 Submit a Question for ab5627

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    Votes: Highest First

    Please let me know if PKC ab5627 can detect all the isoforms partcularly delta or very specific to beta isoform only. If this ab could be used on paraffin embedded formalin fixed tissue? Do you sell smaller packs as samples which could be used for a pil...

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    Thank you for your enquiry. Ab5627 has only been tested on rec. beta II protein. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. We do not routinely offer free or trial size...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"