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Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody (ab5817)

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Overview

Product name

Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody
See all PKC beta 1 + PKC beta 2 products (3) ...

Description

Rabbit polyclonal to PKC beta 1 + PKC beta 2 (phospho T500)

Specificity

This antibody cross-reacts with PKC alpha [pT497] (88% homologous) and partially cross-reacts with PKC gamma [pT514] (63% homologous) and epsilon [pT566] (75% homologous), as determined by peptide competition experiments.

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse

Immunogen

Synthetic phosphopeptide derived from a region of human PKC beta 1 & 2 that contains threonine 500.

Positive control

K562 cells treated with PMA, a phorbol ester.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC beta. The final product is generated by affinity chromatography using a PKC beta-derived peptide that is phosphorylated at threonine 500.

Primary antibody notes

Protein Kinase C beta (PKC beta) is an 80 kDa member of the conventional group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. PKC beta has been implicated in diabetes and carcinogenesis. PKC beta isoforms 1 & 2 are phosphorylated on three sites, threonine 500 in the activation loop, beta 1 threonine 642 (beta 2 641) in the turn loop and beta 1 serine 661 (beta 2 660) in the hydrophobic loop. Phosphorylation of PKC beta 1 & 2 on threonine 500 by PDK1 is a prerequisite for its autophosphoylation and catalytic competence.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody (ab5817)Western blot - Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody (ab5817) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab5817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/1000. Punctate staining observed.
WB: 1/1000. Detects a band of approximately 80 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.

Post-translational
modifications

Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.

Cellular localization

Cytoplasm. Nucleus. Membrane.

Target information above from: UniProt accessionP05771 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • KPCB_HUMAN antibody
  • PKC beta antibody
  • PKC-B antibody
  • PKC-beta antibody
  • PKCB antibody
  • PKCB1 antibody
  • PKCB2 antibody
  • Prkcb antibody
  • PRKCB I antibody
  • PRKCB II antibody
  • PRKCB1 antibody
  • PRKCB2 antibody
  • Protein kinase C beta 1 antibody
  • Protein kinase C beta 2 antibody
  • Protein kinase C beta antibody
  • Protein kinase C beta type antibody
see all

Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody images:

  Western blot - Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody (ab5817)

Western blot - Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody (ab5817)

Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5817 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to PKC beta 1 & 2 [pT500] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

References for Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody (ab5817)

This product has been referenced in:

  • Spalding AC  et al. Inhibition of protein kinase Cbeta by enzastaurin enhances radiation cytotoxicity in pancreatic cancer. Clin Cancer Res 13:6827-33 (2007).Read more (PubMed: 18006785) »

See 1 publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"