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Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
  • PurityWhole antiserum
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Predicted molecular weight: 80 kDa. (using alkaline phosphatase or horseradish peroxidase).

With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
ELISA ELISA: Use at an assay dependent dilution.
ICC ICC: 1/250. (frozen) (using alkaline phosphatase or horseradish peroxidase).
IHC-Fr IHC-Fr: Use at an assay dependent dilution.

Target

  • FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • Cellular localizationCytoplasm. Nucleus. Membrane.

    • Target information above from: UniProt accession P05771 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      KPCB_HUMAN antibodyMGC41878 antibodyPKC B antibody
      PKC beta antibodyPKC-B antibodyPKC-beta antibodyPKCB antibodyPRKC B antibodyPRKC B1 antibodyPRKCB antibodyPRKCB I antibodyPRKCB1 antibodyPRKCB2 antibodyProtein kinase C beta 1 antibodyProtein kinase C beta antibodyProtein kinase C beta type antibody
    see all

Anti-PKC beta 1 antibody images

  • Western blot
    Western blot
    Anti-PKC beta 1 antibody (ab4132) at 1/1000 dilution + PKC beta 1 protein (Active) (ab60840) at 0.1 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • Western blot - PKC beta 1 antibody (ab4132)
    Western blot - PKC beta 1 antibody (ab4132)
    All lanes : Anti-PKC beta 1 antibody (ab4132) at 1/1000 dilution

    Lane 1 : 20ug BxPC3 whole cell lysate
    Lane 2 : 10pg recombinant human PKCbeta1
    Lane 3 : 3pg recombinant human PKCbeta1
    Lane 4 : 1pg recombinant human PKCbeta1

    Secondary
    HRP conjugated mouse anti-rabbit.
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 80 kDa
    Additional bands at : 50 kDa (possible non-specific binding),60 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-PKC beta 1 antibody (ab4132)

This product has been referenced in:
  • Makary S  et al. Differential protein kinase C isoform regulation and increased constitutive activity of acetylcholine-regulated potassium channels in atrial remodeling. Circ Res 109:1031-43 (2011). WB ; Dog . Read more (PubMed: 21903936) »
  • Hsieh TJ  et al. Upregulation of osteopontin gene expression in diabetic rat proximal tubular cells revealed by microarray profiling. Kidney Int 69:1005-15 (2006). Read more (PubMed: 16528250) »

See all 2 Publications for this product

Publishing research using ab4132 ? Please let us know so that we can cite the reference in this datasheet

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Western blot abreview for Anti-PKC beta 1 antibody

 Good
Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (BxPC3)
Loading amount 20 µg
Specification BxPC3
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Read More
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Submitted Aug 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"