Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
  • PurityWhole antiserum
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 80 kDa. (using alkaline phosphatase or horseradish peroxidase).
With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
ELISA Use at an assay dependent dilution.
ICC 1/250. (frozen) (using alkaline phosphatase or horseradish peroxidase).
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • Cellular localizationCytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCB_HUMAN antibody
    • MGC41878 antibody
    • PKC B antibody
    • PKC beta antibody
    • PKC-B antibody
    • PKC-beta antibody
    • PKCB antibody
    • PRKC B antibody
    • PRKC B1 antibody
    • PRKCB antibody
    • PRKCB I antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • Protein kinase C beta 1 antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    • protein kinase C, beta 1 polypeptide antibody
    • protein kinase C, beta-1 antibody
    see all

Anti-PKC beta 1 antibody images

  • Anti-PKC beta 1 antibody (ab4132) at 1/1000 dilution + Active human PKC beta 1 full length protein (ab60840) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • All lanes : Anti-PKC beta 1 antibody (ab4132) at 1/1000 dilution

    Lane 1 : 20ug BxPC3 whole cell lysate
    Lane 2 : 10pg recombinant human PKCbeta1
    Lane 3 : 3pg recombinant human PKCbeta1
    Lane 4 : 1pg recombinant human PKCbeta1

    Secondary
    HRP conjugated mouse anti-rabbit.
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 80 kDa
    Additional bands at : 50 kDa (possible non-specific binding),60 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-PKC beta 1 antibody (ab4132)

This product has been referenced in:
  • Qu C  et al. Trimeric G protein-CARMA1 axis links smoothened, the hedgehog receptor transducer, to NF-?B activation in diffuse large B-cell lymphoma. Blood 121:4718-28 (2013). Read more (PubMed: 23632891) »
  • Makary S  et al. Differential protein kinase C isoform regulation and increased constitutive activity of acetylcholine-regulated potassium channels in atrial remodeling. Circ Res 109:1031-43 (2011). WB ; Dog . Read more (PubMed: 21903936) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (J774 macrophage)
Specification J774 macrophage
Treatment siRNA for 48h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (BxPC3)
Loading amount 20 µg
Specification BxPC3
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

Submitted Aug 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"