Anti-PKC beta 1 (phospho T642) antibody (ab5782)

Overview

• Product nameAnti-PKC beta 1 (phospho T642) antibodySee all PKC beta 1 primary antibodies ...
• Description
  Rabbit polyclonal to PKC beta 1 (phospho T642)
• SpecificityThis antibody does not react with PKC beta 2 [pT641], alpha [pT638], nu [pT655], epsilon [pT710], iota [pT555], eta [pT655], or zeta [pT560] as determined by peptide competition experiments.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Synthetic phosphopeptide derived from a region of human PKC beta 1 that contains threonine 642.

• Positive controlK562 cells treated with PMA, a phorbol ester.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC beta 1. The final product is generated by affinity chromatography using a PKC beta 1-derived peptide that is phosphorylated at threonine 642.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKC

Applications

Target

• FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 C2 domain.
  Contains 2 phorbol-ester/DAG-type zinc fingers.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
• Cellular localizationCytoplasm. Nucleus. Membrane.

Target information above from: UniProt accession P05771 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5579 Human
  • Entrez Gene: 5579 Human
  • Entrez Gene: 25023 Rat
  • Omim: 176970 Human
  • SwissProt: P05771 Human
  • SwissProt: P05771 Human
  • SwissProt: P68403 Rat
  • Unigene: 460355 Human

  • Unigene: 91118 Rat

  see all

• Alternative names
  KPCB_HUMAN antibodyMGC41878 antibodyPKC B antibody

  PKC beta antibodyPKC-B antibodyPKC-beta antibodyPKCB antibodyPRKC B antibodyPRKC B1 antibodyPRKCB antibodyPRKCB I antibodyPRKCB1 antibodyPRKCB2 antibodyProtein kinase C beta 1 antibodyProtein kinase C beta antibodyProtein kinase C beta type antibody

  see all

Anti-PKC beta 1 (phospho T642) antibody images

• 

  Western blot - Anti-PKC beta 1 (phospho T642) antibody (ab5782)
  Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (ë) phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5782 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to PKC beta I [pT642] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.