Anti-PKC beta 1 (phospho T642) antibody (ab75657)

Overview

• Product nameAnti-PKC beta 1 (phospho T642) antibodySee all PKC beta 1 primary antibodies ...
• Description
  Rabbit polyclonal to PKC beta 1 (phospho T642)
• Specificityab75657 detects endogenous levels of PKC beta 1 only when phosphorylated at threonine 642.
• Tested applicationsICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic phosphopeptide derived from human PKC beta 1 around the phosphorylation site of threonine 642 (E-L-T^P-P-T).

• Positive controlHuman lung carcinoma tissue. Extracts from K562 cells treated with PMA (1ng/ml, 10min).

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab75657 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKC

Applications

Target

• FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 C2 domain.
  Contains 2 phorbol-ester/DAG-type zinc fingers.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
• Cellular localizationCytoplasm. Nucleus. Membrane.

Target information above from: UniProt accession P05771 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5579 Human
  • Entrez Gene: 5579 Human
  • Entrez Gene: 18751 Mouse
  • Entrez Gene: 25023 Rat
  • Omim: 176970 Human
  • SwissProt: P05771 Human
  • SwissProt: P05771 Human
  • SwissProt: P68404 Mouse

  • SwissProt: P68403 Rat
  • Unigene: 460355 Human
  • Unigene: 207496 Mouse
  • Unigene: 446371 Mouse
  • Unigene: 91118 Rat

  see all

• Alternative names
  KPCB_HUMAN antibodyMGC41878 antibodyPKC B antibody

  PKC beta antibodyPKC-B antibodyPKC-beta antibodyPKCB antibodyPRKC B antibodyPRKC B1 antibodyPRKCB antibodyPRKCB I antibodyPRKCB1 antibodyPRKCB2 antibodyProtein kinase C beta 1 antibodyProtein kinase C beta antibodyProtein kinase C beta type antibody

  see all

Anti-PKC beta 1 (phospho T642) antibody images

• 

  Western blot - PKC beta 1 (phospho T642) antibody (ab75657)
  All lanes : Anti-PKC beta 1 (phospho T642) antibody (ab75657) at 1/500 dilution
  
  Lane 1 : K562 cells, untreated
  Lane 2 : K562 cells treated with PMA (1ng/ml, 10min)
  
  
  Predicted band size : 77 kDa
  Observed band size : 77 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC beta 1 (phospho T642) antibody (ab75657)
  ab75657 at 1/50 dilution staining PKC beta 1 in human lung carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
• 

  Immunocytochemistry/ Immunofluorescence - PKC beta 1 (phospho T642) antibody (ab75657)

  ab75657 at 1/100 dilution staining PKC beta 1 (phospho T642) in human MCF7 cells by ICC/IF.