Overview

  • Product nameAnti-PKC beta 1 (phospho T642) antibodySee all PKC beta 1 primary antibodies ...
  • Description
    Rabbit polyclonal to PKC beta 1 (phospho T642)
  • Specificityab75657 detects endogenous levels of PKC beta 1 only when phosphorylated at threonine 642.
  • Tested applicationsICC/IF, WB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human PKC beta 1 around the phosphorylation site of threonine 642 (E-L-TP-P-T).

  • Positive control
    • Human lung carcinoma tissue. Extracts from K562 cells treated with PMA (1ng/ml, 10min).

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab75657 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab75657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • Cellular localizationCytoplasm. Nucleus. Membrane.
  • Target information above from: UniProt accession P05771 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • KPCB_HUMAN antibody
    • MGC41878 antibody
    • PKC B antibody
    • PKC beta antibody
    • PKC-B antibody
    • PKC-beta antibody
    • PKCB antibody
    • PRKC B antibody
    • PRKC B1 antibody
    • PRKCB antibody
    • PRKCB I antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • Protein kinase C beta 1 antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    see all

Anti-PKC beta 1 (phospho T642) antibody images

  • All lanes : Anti-PKC beta 1 (phospho T642) antibody (ab75657) at 1/500 dilution

    Lane 1 : K562 cells, untreated
    Lane 2 : K562 cells treated with PMA (1ng/ml, 10min)


    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • ab75657 at 1/50 dilution staining PKC beta 1 in human lung carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • ab75657 at 1/100 dilution staining PKC beta 1 (phospho T642) in human MCF7 cells by ICC/IF.

References for Anti-PKC beta 1 (phospho T642) antibody (ab75657)

This product has been referenced in:
  • Qu C  et al. Trimeric G protein-CARMA1 axis links smoothened, the hedgehog receptor transducer, to NF-?B activation in diffuse large B-cell lymphoma. Blood 121:4718-28 (2013). Read more (PubMed: 23632891) »
  • Feng J  et al. Changes in microvascular reactivity after cardiopulmonary bypass in patients with poorly controlled versus controlled diabetes. Circulation 126:S73-80 (2012). IHC ; Human . Read more (PubMed: 22965996) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"