Anti-PKC beta 2 antibody [Y125] (ab32026)

Overview

• Product nameAnti-PKC beta 2 antibody [Y125]See all PKC beta 2 primary antibodies ...
• Description
  Rabbit monoclonal [Y125] to PKC beta 2
• Specificityab32026 recognises PKC beta 2, it does not cross react with human PKC beta 1. Immunogen's homology with PKC alpha is 77.8%. Cross-reactivity with PKC alpha has not been tested.
• Tested applicationsWB, IHC-P, ICC/IF, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Cow, Pig
• Immunogen

  Synthetic peptide corresponding to residues near C terminus of human PKC beta 2

• Epitopeab32026 reacts with an epitope located in the region near the C terminus of PKC beta 2.
• Positive controlK562 cell lysate, HeLa cell lysate and human breast carcinoma
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberY125
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKC

Applications

Target

• FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 C2 domain.
  Contains 2 phorbol-ester/DAG-type zinc fingers.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
• Cellular localizationCytoplasm. Nucleus. Membrane.

Target information above from: UniProt accession P05771 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5579 Human
  • Entrez Gene: 18751 Mouse
  • Entrez Gene: 25023 Rat
  • Omim: 176970 Human
  • SwissProt: P05126 Cow
  • SwissProt: P05771 Human
  • SwissProt: P68404 Mouse
  • SwissProt: P68403 Rat

  • Unigene: 460355 Human
  • Unigene: 207496 Mouse
  • Unigene: 446371 Mouse
  • Unigene: 91118 Rat

  see all

• Alternative names
  KPCB_HUMAN antibodyMGC41878 antibodyPKC B2 antibody

  PKC beta antibodyPKC beta antibodyPKC beta II antibodyPKC-B antibodyPKC-beta antibodyPKCB antibodyPKCB antibodyPKCB2 antibodyPKCB2 antibodyPKCBII antibodyPRKC B2 antibodyPRKCB antibodyPRKCB antibodyPRKCB II antibodyPRKCB1 antibodyPRKCB2 antibodyPRKCB2 antibodyProtein kinase C beta 1 polypeptide antibodyProtein kinase C beta 1 polypeptide antibodyProtein kinase C beta 2 antibodyProtein kinase C beta 2 antibodyProtein kinase C beta antibodyProtein kinase C beta type antibody

  see all

Anti-PKC beta 2 antibody [Y125] images

• 

  Western blot - PKC beta 2 antibody [Y125] (ab32026)
  Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + K562 cell lysate
  
  Predicted band size : 77 kDa
  Observed band size : 77 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - PKC beta 2 antibody [Y125] (ab32026)
  ab32026, at a 1/100 dilution, staining human PKC beta 2 in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue
• 

  Immunofluorescence - PKC beta 2 antibody [Y125] (ab32026)
  ab32026, at a 1/250 dilution, staining human PKC beta 2 in HeLa cells by Immunoflourescence
• 

  Flow Cytometry-Anti-PKC beta 2 antibody [Y125](ab32026)
  Overlay histogram showing HeLa cells stained with ab32026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32026, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.