Overview

  • Product nameAnti-PKC beta 2 antibody [Y125]See all PKC beta 2 primary antibodies ...
  • Description
    Rabbit monoclonal [Y125] to PKC beta 2
  • Specificityab32026 recognises PKC beta 2, it does not cross react with human PKC beta 1. Immunogen's homology with PKC alpha is 77.8%. Cross-reactivity with PKC alpha has not been tested.
  • Tested applicationsWB, IHC-P, ICC/IF, Flow Cyt, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to residues near C terminus of human PKC beta 2

  • Epitopeab32026 reacts with an epitope located in the region near the C terminus of PKC beta 2.
  • Positive control
    • K562 cell lysate, HeLa cell lysate and human breast carcinoma
  • General notes

    Produced under U.S. Patent No. 5,675,063.

     

    A trial size is available for this product.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • Clone numberY125
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab32026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-P Use at an assay dependent dilution.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/50.
IP 1/60.

Target

  • FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • Cellular localizationCytoplasm. Nucleus. Membrane.
  • Target information above from: UniProt accession P05771 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • KPCB_HUMAN antibody
    • MGC41878 antibody
    • PKC B2 antibody
    • PKC beta antibody
    • PKC beta antibody
    • PKC beta II antibody
    • PKC-B antibody
    • PKC-beta antibody
    • PKCB antibody
    • PKCB antibody
    • PKCB2 antibody
    • PKCB2 antibody
    • PKCBII antibody
    • PRKC B2 antibody
    • PRKCB antibody
    • PRKCB antibody
    • PRKCB II antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • PRKCB2 antibody
    • Protein kinase C beta 1 polypeptide antibody
    • Protein kinase C beta 1 polypeptide antibody
    • Protein kinase C beta 2 antibody
    • Protein kinase C beta 2 antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    see all

Anti-PKC beta 2 antibody [Y125] images

  • Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + K562 cell lysate

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • ab32026, at a 1/100 dilution, staining human PKC beta 2 in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue
  • ab32026, at a 1/250 dilution, staining human PKC beta 2 in HeLa cells by Immunoflourescence
  • Overlay histogram showing HeLa cells stained with ab32026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32026, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-PKC beta 2 antibody [Y125] (ab32026)

This product has been referenced in:
  • Qu C  et al. Trimeric G protein-CARMA1 axis links smoothened, the hedgehog receptor transducer, to NF-?B activation in diffuse large B-cell lymphoma. Blood 121:4718-28 (2013). Read more (PubMed: 23632891) »
  • Maharaj NP  et al. Conventional protein kinase C-a (PKC-a) and PKC-ß negatively regulate RIG-I antiviral signal transduction. J Virol 86:1358-71 (2012). WB . Read more (PubMed: 22114345) »

See all 4 Publications for this product

Product Wall

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This antibody has been tested in IP so it will more likely to work in Co-IP also. However we cannot confirm it because we do not have tested data. The experimental conditions have to be determined by the end user...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Loading amount 100000 cells
Specification MCF7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (GLC16 Cells)
Loading amount 40000 cells
Specification GLC16 Cells
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"