Anti-PKC beta 2 antibody (ab4138)

Overview

  • Product nameAnti-PKC beta 2 antibody
    See all PKC beta 2 primary antibodies
  • Description
    Rabbit polyclonal to PKC beta 2
  • Tested applicationsELISA, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human, all Mammals
  • Immunogen

    Synthetic peptide:

    SFVNSEFLKPEVKS

    , corresponding to amino acids 660-673 of Rat PKC beta 2.

  • Positive control
    • Brain and spleen.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
  • PurityWhole antiserum
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC
IHC-Fr
WB
  • Application notesELISA: assay dependent.
    Immunocytochemistry (frozen): 1/250 (using alkaline phosphatase or horseradish peroxidase).
    Western blot (enhanced chemiluminescence): 1/250 (using alkaline phosphatase or horseradish peroxidase). Predicted molecular weight: 80 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
  • Target

    • FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
    • Cellular localizationCytoplasm. Nucleus. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCB_HUMAN antibody
      • MGC41878 antibody
      • PKC B2 antibody
      • PKC beta antibody
      • PKC beta II antibody
      • PKC-B antibody
      • PKC-beta antibody
      • PKCB antibody
      • PKCB2 antibody
      • PKCBII antibody
      • PRKC B2 antibody
      • PRKCB antibody
      • PRKCB II antibody
      • PRKCB1 antibody
      • PRKCB2 antibody
      • Protein kinase C beta 1 polypeptide antibody
      • Protein kinase C beta 2 antibody
      • Protein kinase C beta antibody
      • Protein kinase C beta type antibody
      see all

    References for Anti-PKC beta 2 antibody (ab4138)

    ab4138 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for getting back to me. Thank you also for trying my suggestions. I am sorry that they did not lead to any improvements to the results that you have been obtaining. As mentioned previously unfortunately we cannot guarantee this antibody to wo...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. As my colleague has explained unfortunately this antibody is yet to be tested using mouse and human tissue. However, I would predict its react...

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    Thank you for the protocol details that you have provided and I'm sorry to hear that you're experiencing difficulty with ab4138. I noticed that you are using human and mouse samples. Ab4138 has been tested for cross-reactivity with rat PKC beta 2 and a...

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    Thank you for your enquiry and for your detailed protocol. This antibody recognizes Rat PKC beta 2 and the predicted molecular weight is kDa. We would like to emphasize the fact that this antibody has only been tested on rat samples. Since you ar...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"