RabMAb

Anti-PKC beta 2 (phospho S660) antibody [EP1902Y] (ab75837)

Overview

  • Product nameAnti-PKC beta 2 (phospho S660) antibody [EP1902Y]
    See all PKC beta 2 primary antibodies
  • Description
    Rabbit monoclonal [EP1902Y] to PKC beta 2 (phospho S660)
  • Specificityab75837 detects PKC beta 2 phosphorylated at serine 660 as well as PKCD phosphorylated on serine 664 due to high homology of the immunogen sequence.
  • Tested applicationsIHC-P, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 660 of human PKC beta 2

  • Positive control
    • Jurkat cell lysates, untreated or treated with AP; human breast carcinoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • Clonality Monoclonal
  • Clone numberEP1902Y
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab75837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/50000 - 1/200000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
    • Cellular localizationCytoplasm. Nucleus. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCB_HUMAN antibody
      • PKC Beta antibody
      • PKC-B antibody
      • PKC-beta antibody
      • PKCB antibody
      • Prkcb antibody
      • PRKCB II antibody
      • PRKCB2 antibody
      • Protein kinase C beta antibody
      • Protein kinase C beta type antibody
      see all

    Anti-PKC beta 2 (phospho S660) antibody [EP1902Y] images

    • All lanes : Anti-PKC beta 2 (phospho S660) antibody [EP1902Y] (ab75837) at 1/100000 dilution

      Lane 1 : Jurkat cell lysates, untreated
      Lane 2 : Jurkat cell lysates, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 77 kDa
      Observed band size : 77 kDa
    • ab75837, at a 1/100 dilution, staining PKC beta 2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

    References for Anti-PKC beta 2 (phospho S660) antibody [EP1902Y] (ab75837)

    This product has been referenced in:
    • Lin G  et al. Complex regulation of PKCß2 and PDK-1/AKT by ROCK2 in diabetic heart. PLoS One 9:e86520 (2014). WB ; Rat . Read more (PubMed: 24466133) »
    • Qu C  et al. Trimeric G protein-CARMA1 axis links smoothened, the hedgehog receptor transducer, to NF-?B activation in diffuse large B-cell lymphoma. Blood 121:4718-28 (2013). Read more (PubMed: 23632891) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Sample Cow Cell lysate - other (Cerebral microvascular endothelial cells)
    Gel Running Conditions Reduced Denaturing (8% Bis-Tris)
    Loading amount 10 µg
    Specification Cerebral microvascular endothelial cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 25°C
    Username

    Nicholas Klug

    Verified customer

    Submitted Jun 26 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"