![Western blot - PKC delta antibody [EP1484Y] (ab52763) image](#ab52763.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Overview
Product name
Anti-PKC delta antibody [EP1484Y]
See all PKC delta products (21) ...
Description
Rabbit monoclonal [EP1484Y] to PKC delta
Specificity
This antibody reacts with PKC delta.
Tested applications
WB, Flow Cyt, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues near the C terminus of human PKC delta.
Positive control
WB: Hela cell lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1484Y
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> MAPK pathway
Neuroscience >> Neurology process >> Neuroendocrinology >> Prolactin
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
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Western blot - PKC delta antibody [EP1484Y] (ab52763)
![Western blot - PKC delta antibody [EP1484Y] (ab52763) image](/ps/datasheet/Images/52/ab52763/ab52763.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
Flow Cyt
Flow Cyt: 1/15Flow Cyt: 1/15
IHC-P: 1/50(Antigen retrieval is recommended.)
ICC/IF
ICC/IF: 1/50 - 1/100.ICC/IF: 1/50 - 1/100.
Application notes
Is unsuitable for or IP.
Target
Relevance
Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.
Cellular localization
Cytoplasmic
Alternative names
- D14Ertd420e antibody
- MAY 1 antibody
- MAY1 antibody
see all
Database links
- Entrez Gene: 5580 Human
- Omim: 176977 Human
- SwissProt: Q05655 Human
- Unigene: 155342 Human
Anti-PKC delta antibody [EP1484Y] images:
Western blot - PKC delta antibody [EP1484Y] (ab52763)
Anti-PKC delta antibody [EP1484Y] (ab52763) at 1/500 dilution + Hela cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 77 kDa
Observed band size : 77 kDa
References for Anti-PKC delta antibody [EP1484Y] (ab52763)
ab52763 has not yet been referenced specifically in any publications.
Publishing research using ab52763? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"