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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Overview
Product name
Anti-PKC delta antibody
See all PKC delta products (21) ...
Description
Rabbit polyclonal to PKC delta
Tested applications
WB, ELISA, IHC-Fr, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
all Mammals
Immunogen
Synthetic peptide: SFVNPKYEQFLE, corresponding to amino acids 662-673 of Rat PKC delta
SFVNPKYEQF LE
Positive control
rat cerebellar lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> MAPK pathway
Neuroscience >> Neurology process >> Neuroendocrinology >> Prolactin
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
IHC-Fr
ICC
Application notes
ELISA: Use at an assay dependent dilution.
ICC: 1/250.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/250. Predicted molecular weight: 81 kDa. Use lysis buffer made of 20
mM Tris-HCI, pH 7.5, 0 .25 M sucrose, 10 mM EGTA, 1 mM EDTA, 10
mM ß-mercaptoethanol, 1 mM PMSF, 0.02% leupeptin, and 0.1% Triton
X-100. Incubate the homogenate for 1 h at 4°C and centrifuge at
100,000 g for 30 min at 4°C. (see Wetsel et al reference).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.
Cellular localization
Cytoplasmic
Alternative names
- D14Ertd420e antibody
- D14Ertd420e antibody
- MAY 1 antibody
see all
Database links
- Entrez Gene: 18753 Mouse
- Entrez Gene: 170538 Rat
- SwissProt: P28867 Mouse
- SwissProt: P09215 Rat
- Unigene: 98279 Rat
References for Anti-PKC delta antibody (ab4142)
This product has been referenced in:
- Son NHet al. PPAR?-induced cardiolipotoxicity in mice is ameliorated by PPARa deficiency despite increases in fatty acid oxidation. J Clin Invest 120:3443-54 (2010). WB; Mouse.Read more (PubMed: 20852389) »
See 1 publication for this product
Publishing research using ab4142? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"