PKC delta antibody (ab4142)

Product Name 

PKC delta antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PKC delta

Immunogen 

Synthetic peptide: SFVNPKYEQFLE, corresponding to amino acids 662-673 of Rat PKC delta
BLAST 'SFVNPKYEQFLE' with or

Reacts with 

Rat

Predicted to react 
(species key)

Ms, Mamm
(Due to sequence homology)

Tested applications 
(see key)

ELISA, ICC, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: 1/250.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/250. Predicted molecular weight: 81 kDa. Use lysis buffer made of 20
mM Tris-HCI, pH 7.5, 0 .25 M sucrose, 10 mM EGTA, 1 mM EDTA, 10
mM ß-mercaptoethanol, 1 mM PMSF, 0.02% leupeptin, and 0.1% Triton
X-100. Incubate the homogenate for 1 h at 4°C and centrifuge at
100,000 g for 30 min at 4°C. (see Wetsel et al reference).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

rat cerebellar lysate

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> MAPK pathway
Neuroscience >> Neurology process >> Neuroendocrinology >> Prolactin
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC

Relevance 

Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.05% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for PKC delta antibody (ab4142)

  This product has been used in: (two most recent references)

Mischak H et al. Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta. J Biol Chem 268:20110-5 (1993). PubMed: 8376369

Wetsel WC et al. Tissue and cellular distribution of the extended family of protein kinase C isoenzymes. J Cell Biol 117:121-33 (1992). PubMed: 1556149
If you publish research using ab4142 please let us know so that we can cite the reference on this datasheet.

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