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ab27714 |
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ab4143 has been referenced in 2 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 81 kDa
Total protein was isolated by direct lysis of adherent cells or isolated from cell pellets washed with ice-cold PBS pH 7.4, using cell lysis buffer. 40µg of protein/line, as determined by a Bradford assay, were resolved by SDS-PAGE, transferred to a nitrocellulose membrane, blocked and hybridized with ab4143, followed by development with infrared IR dye 700CW- and/or 800CW-labeled secondary antibodies.
Image from Tomilov AA et al, J Biol Chem. 2010 Jan 8;285(2):1153-65. Epub 2009 Nov 5, Fig 9.
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