Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Overview
Product name
Anti-PKC delta antibody
See all PKC delta products (21) ...
Description
Rabbit polyclonal to PKC delta
Specificity
This antibody has only been tested against the recombinant protein and not against the endogenous protein.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
all Mammals
Immunogen
Synthetic peptide: SAFAGFSFVNPKFELLED, corresponding to amino acids 658-676 of Human PKC delta.
SAFAGFSFVN PKFELLED
Positive control
recombinant protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> MAPK pathway
Neuroscience >> Neurology process >> Neuroendocrinology >> Prolactin
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
-
Western blot - PKC delta antibody (ab4143)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Western blot: 1/100 (enhanced chemiluminescence). Predicted molecular weight: 81 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
If a less sensitive detecting system, such as alkaline phosphatase, is to be employed, the dilution of the antibody should be decreased.
Target
Relevance
Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.
Cellular localization
Cytoplasmic
Alternative names
- D14Ertd420e antibody
- D14Ertd420e antibody
- MAY 1 antibody
see all
Database links
- Entrez Gene: 5580 Human
- Entrez Gene: 18753 Mouse
- Entrez Gene: 170538 Rat
- Omim: 176977 Human
- SwissProt: Q05655 Human
- SwissProt: P28867 Mouse
- SwissProt: P09215 Rat
- Unigene: 155342 Human
see all
Anti-PKC delta antibody images:
Western blot - PKC delta antibody (ab4143)
Predicted band size : 81 kDa
Total protein was isolated by direct lysis of adherent cells or isolated from cell pellets washed with ice-cold PBS pH 7.4, using cell lysis buffer. 40µg of protein/line, as determined by a Bradford assay, were resolved by SDS-PAGE, transferred to a nitrocellulose membrane, blocked and hybridized with ab4143, followed by development with infrared IR dye 700CW- and/or 800CW-labeled secondary antibodies.
Image from Tomilov AA et al, J Biol Chem. 2010 Jan 8;285(2):1153-65. Epub 2009 Nov 5, Fig 9.
References for Anti-PKC delta antibody (ab4143)
This product has been referenced in:
- Tomilov AAet al. Decreased superoxide production in macrophages of long-lived p66Shc knock-out mice. J Biol Chem 285:1153-65 (2010). WB; Mouse.Read more (PubMed: 19892704) »
- Son NHet al. PPAR?-induced cardiolipotoxicity in mice is ameliorated by PPARa deficiency despite increases in fatty acid oxidation. J Clin Invest 120:3443-54 (2010). WB; Mouse.Read more (PubMed: 20852389) »
See all 2 publications for this product
Publishing research using ab4143? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"