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Anti-PKC delta antibody (ab47473)

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Overview

Product name

Anti-PKC delta antibody
See all PKC delta products (21) ...

Description

Rabbit polyclonal to PKC delta

Specificity

This antibody detects endogenous levels of total PKCd protein.

Tested applications

WB, IHC-P, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthesized non-phosphopeptide derived from human PKC delta around the phosphorylation site of serine 645 (R-L-SP-Y-S).

Positive control

MCF7 cells and human breast carcinoma tissue

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> MAPK pathway
Neuroscience >> Neurology process >> Neuroendocrinology >> Prolactin
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC

  • Western blot - PKC delta antibody (ab47473)Western blot - PKC delta antibody (ab47473) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - PKC delta antibody (ab47473)Immunohistochemistry (Paraffin-embedded sections) - PKC delta antibody (ab47473) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab47473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

Target

Relevance

Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Cellular localization

Cytoplasmic

Alternative names

  • D14Ertd420e antibody
  • MAY 1 antibody
  • MAY1 antibody
  • MGC49908 antibody
  • nPKC delta antibody
  • PCKd antibody
  • PKC d antibody
  • PKC delta antibody
  • PKC-d antibody
  • PKCD antibody
  • PKCdelta antibody
  • PRKC D antibody
  • PRKC delta antibody
  • PRKC-d antibody
  • PRKCd antibody
  • Protein Kinase C delta antibody
  • Protein kinase C delta type antibody
  • Protein Kinase Cdelta antibody
see all

Database links

see all

Anti-PKC delta antibody images:

  Western blot - PKC delta antibody (ab47473)

Western blot - PKC delta antibody (ab47473)

All lanes : Anti-PKC delta antibody (ab47473) at 1/500 dilution

Lane 1 : Extract of MCF7 cells un-treated
Lane 2 : Extract of MCF7 cells with synthetic peptide

Lysates/proteins at 30 µg per lane.


Predicted band size : 77 kDa
Observed band size : 77 kDa

  Immunohistochemistry (Paraffin-embedded sections) - PKC delta antibody (ab47473)

Immunohistochemistry (Paraffin-embedded sections) - PKC delta antibody (ab47473)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of synthetic peptide, using ab47473 at a 1/50 dilution.

References for Anti-PKC delta antibody (ab47473)

This product has been referenced in:

  • Cai Qet al. Protein kinase Cdelta negatively regulates hedgehog signaling by inhibition of Gli1 activity. J Biol Chem 284:2150-8 (2009). IHC-P; Human.Read more (PubMed: 19015273) »

See 1 publication for this product

Publishing research using ab47473? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"