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Product name
Anti-PKC delta (phospho S645) antibody
See all PKC delta products (23) ...
Description
Rabbit polyclonal to PKC delta (phospho S645)
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide derived from the region of human PKC delta that contains serine 645.
Positive control
U937 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated PKC delta peptide. The final product is generated by affinity chromatography using a PKC delta derived peptide that is phosphorylated at serine 645.
Primary antibody notes
Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. In contrast to other PKC isozymes where phosphorylation of the activation loop residue is essential for catalytic activity, phosphorylation of the activation loop threonine 507 of PKC delta appears to modulate, but is not required for, catalytic activity. This phosphorylation may also regulate PKC delta stability and degradation. Autophosphorylation at serine 645 (serine 643 in mouse and rat) is not required for, but may modulate, catalytic activity and receptor dephosphorylation.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> MAPK pathway
Neuroscience >> Neurology process >> Neuroendocrinology >> Prolactin
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 77 kDa.
Target
Function
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
Post-translational
modifications
Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
Cellular localization
Cytoplasm. Membrane.
Target information above from: UniProt accessionQ05655
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D14Ertd420e antibody
- D14Ertd420e antibody
- KPCD_HUMAN antibody
see all
Database links
- Entrez Gene: 5580 Human
- Omim: 176977 Human
- SwissProt: Q05655 Human
- Unigene: 155342 Human
References for Anti-PKC delta (phospho S645) antibody (ab5657)
ab5657 has not yet been referenced specifically in any publications.
Publishing research using ab5657? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"