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Overview

  • Product namePKC epsilon protein (Active)See all PKC epsilon proteins and peptides ...
  • Protein descriptionRecombinant full length PKC epsilon protein (Human), was expressed by baculovirus in Sf9 insect cells using a N-terminal tag, MW 110kDa.
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purification notesPurity: >90% as determined by densitometry.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 25% Glycerol, 0.1M PMSF, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab60847 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution. Kinase assay: Use at an assay dependent dilution. Specific Activity: 1258 nmol/min/mg.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent concentration. Ab60847 can be used as a WB positive control in conjunction with ab63638.

    Protein info

    • Alternative names
        KPCE_HUMANMGC125656MGC125657
        nPKC epsilonnPKC-epsilonPKCEPkceaPRKCEProtein kinase C epsilonProtein kinase C epsilon type
      see all
  • FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications    Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.

    • Target information above from: UniProt accession Q02156 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PKC epsilon protein (Active) images

    • Functional Studies - PKC epsilon protein (Tagged) (ab60847)
      Functional Studies - PKC epsilon protein (Tagged) (ab60847)
      Sample Kinase Activity Plot.
    • SDS-PAGE - PKC epsilon protein (Tagged) (ab60847)
      SDS-PAGE - PKC epsilon protein (Tagged) (ab60847)
      ab60847 on SDS-PAGE, MW ~110 kDa.

    References for PKC epsilon protein (Active) (ab60847)

    ab60847 has not yet been referenced specifically in any publications.

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