PKC epsilon protein (Active) (ab60847)
Constituents: 25% Glycerol, 0.1M PMSF, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, pH 7.5
Our Abpromise guarantee covers the use of ab60847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent dilution. Kinase assay: Use at an assay dependent dilution. Specific Activity: 1258 nmol/min/mg.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|WB||WB: Use at an assay dependent concentration. Ab60847 can be used as a WB positive control in conjunction with ab63638.|
- nPKC epsilonnPKC-epsilonPKCEPkceaPRKCEProtein kinase C epsilonProtein kinase C epsilon type
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
modificationsPhosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
References for PKC epsilon protein (Active) (ab60847)
ab60847 has not yet been referenced specifically in any publications.