Overview

  • Product nameAnti-PKC (phospho T410) antibodySee all PKC primary antibodies ...
  • Description
    Rabbit polyclonal to PKC (phospho T410)
  • SpecificityThis antibody detects endogenous levels of PKCgamma only when phosphorylated at threonine 410.
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthesized phosphopeptide derived from human PKC around the phosphorylation site of threonine 410.

  • Positive control
    • Human lung carcinoma tissue and extract from 3T3 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab51166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 81 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent dilution.

Target

Anti-PKC (phospho T410) antibody images

  • All lanes : Anti-PKC (phospho T410) antibody (ab51166) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells treated with PMA (125ng/ml, 30min) plus phosphopeptide.
    Lane 2 : Extracts from 3T3 cells treated with PMA (125ng/ml, 30min).


    Predicted band size : 81 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab51166 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.

References for Anti-PKC (phospho T410) antibody (ab51166)

ab51166 has not yet been referenced specifically in any publications.

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