Overview

  • Product nameAnti-PKC (phospho T497) antibody
    See all PKC primary antibodies
  • Description
    Rabbit polyclonal to PKC (phospho T497)
  • SpecificityDetects endogenous levels of PKC-pan only when phosphorylated at threonine 497. Recognises all isoforms.
  • Tested applicationsWB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of threonine 497 (T-S-TP-F-C).

  • Positive control
    • Breast carcinoma. NIH/3T3 cells.

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab59411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.
ICC/IF Use a concentration of 1 µg/ml.

Target

Anti-PKC (phospho T497) antibody images

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue, using ab59411 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.
  • ICC/IF image of ab59411 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59411, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PKC (phospho T497) antibody (ab59411) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 81 kDa
    Observed band size : 81 kDa


    Exposure time : 8 minutes

References for Anti-PKC (phospho T497) antibody (ab59411)

ab59411 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"