Anti-PKC theta antibody [EPR1488Y] (ab52494)

Overview

• Product nameAnti-PKC theta antibody [EPR1488Y]See all PKC theta primary antibodies ...
• Description
  Rabbit monoclonal [EPR1488Y] to PKC theta
• SpecificityThis antibody detects both phosphorylated (pT538) and unphosphorylated PKC theta
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues surrounding threonine 538 of human PKC theta

• Positive controlJurkat cell lysate
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR1488Y
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKC

Applications

Target

• FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
  PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
• Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 C2 domain.
  Contains 2 phorbol-ester/DAG-type zinc fingers.
  Contains 1 protein kinase domain.
• DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
• Post-translational
  modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.

Target information above from: UniProt accession Q04759 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5588 Human
  • Entrez Gene: 5588 Human
  • Entrez Gene: 18761 Mouse
  • Entrez Gene: 85420 Rat
  • Omim: 600448 Human
  • SwissProt: Q04759 Human
  • SwissProt: Q04759 Human
  • SwissProt: Q02111 Mouse

  • SwissProt: Q9WTQ0 Rat
  • Unigene: 498570 Human
  • Unigene: 329993 Mouse
  • Unigene: 225125 Rat

  see all

• Alternative names
  KPCT_HUMAN antibodyMGC126514 antibodyMGC141919 antibody

  nPKC theta antibodynPKC-theta antibodynPKCtheta antibodyOTTHUMP00000043364 antibodyOTTHUMP00000043365 antibodyPKC 0 antibodyPKC0 antibodyPRKCQ antibodyPRKCT antibodyProtein kinase C theta antibodyProtein kinase C theta type antibodyProtein Kinase Ctheta antibody

  see all

Anti-PKC theta antibody [EPR1488Y] images

• 

  Western blot - PKC theta antibody [EPR1488Y] (ab52494)
  Anti-PKC theta antibody [EPR1488Y] (ab52494) at 1/500 dilution + Jurkat cell lysate at 10 µg
  
  Secondary
  HRP labeled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 85 kDa
  Observed band size : 85 kDa