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Anti-PKC zeta antibody [EP1490(2)] (ab108970)

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Overview

Product name

Anti-PKC zeta antibody [EP1490(2)]
See all PKC zeta products (13) ...

Description

Rabbit monoclonal [EP1490(2)] to PKC zeta

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to residues in Human PKC zeta (UniProt Q05513).

Positive control

293T, HT-29 and fetal liver lysates

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1490(2)

Isotype

IgG1

Research areas

Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC

  • Western blot - PKC zeta antibody [EP1490(2)] (ab108970)Western blot - PKC zeta antibody [EP1490(2)] (ab108970) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab108970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for ,Flow Cyt,ICC,IHC-P or IP.

Target

Function

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Tissue specificity

Expressed in brain, and to a lesser extent in lung, kidney and testis.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.

Domain

The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG).

Post-translational
modifications

CDH5 is required for its phosphorylation at Thr-410.

Cellular localization

Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.

Target information above from: UniProt accessionQ05513 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 14-3-3-zetaisoform antibody
  • AI098070 antibody
  • aPKCzeta antibody
  • C80388 antibody
  • EC 2.7.11.13 antibody
  • KPCZ_HUMAN antibody
  • nPKC zeta antibody
  • nPKC-zeta antibody
  • OTTHUMP00000001368 antibody
  • OTTHUMP00000044160 antibody
  • PKC 2 antibody
  • PKC ZETA antibody
  • PKC2 antibody
  • Pkcz antibody
  • PKCZETA antibody
  • PKM-zeta, included antibody
  • PRKCZ antibody
  • Protein kinase C zeta antibody
  • Protein kinase C zeta form antibody
  • Protein kinase C zeta type antibody
  • r14-3-3 antibody
  • R74924 antibody
  • zetaPKC antibody
see all

Database links

Anti-PKC zeta antibody [EP1490(2)] images:

  Western blot - PKC zeta antibody [EP1490(2)] (ab108970)

Western blot - PKC zeta antibody [EP1490(2)] (ab108970)

All lanes : Anti-PKC zeta antibody [EP1490(2)] (ab108970) at 1/1000 dilution

Lane 1 : 293T lysate
Lane 2 : HT-29 lysate
Lane 3 : Fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 74 kDa
Observed band size : 74 kDa

References for Anti-PKC zeta antibody [EP1490(2)] (ab108970)

ab108970 has not yet been referenced specifically in any publications.

Publishing research using ab108970? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"