Anti-PKLR antibody (ab96604)
Key features and details
- Rabbit polyclonal to PKLR
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKLR antibody
See all PKLR primary antibodies -
Description
Rabbit polyclonal to PKLR -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within the N terminal amino acids 1-230 of Human PKLR.
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Raji cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Plays a key role in glycolysis. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Involvement in disease
Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. -
Sequence similarities
Belongs to the pyruvate kinase family. - Information by UniProt
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Database links
- Entrez Gene: 539579 Cow
- Entrez Gene: 5313 Human
- Entrez Gene: 18770 Mouse
- Entrez Gene: 24651 Rat
- Omim: 609712 Human
- SwissProt: P30613 Human
- SwissProt: P53657 Mouse
- SwissProt: P12928 Rat
see all -
Alternative names
- EC 2.7.1.40 antibody
- KPYR_HUMAN antibody
- L-PK antibody
see all
Images
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All lanes : Anti-PKLR antibody (ab96604) at 1/5000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab96604 at 1:200 dilution. Image 2: Merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96604 has not yet been referenced specifically in any publications.