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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-PKM2 antibody
See all PKM2 products (8) ...
Description
Rabbit polyclonal to PKM2
Tested applications
ICC/IF, ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Monkey
Immunogen
KLH conjugated synthetic peptide selected from the C-terminal region of human PKM2.
NFAMNVGKAR GFFKK
Positive control
HeLa cell lysate, Human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. Ab38237 recognizes the M1/M2 isoform of pyruvate kinase
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500.Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Target
Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities
Belongs to the pyruvate kinase family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated.
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
Target information above from: UniProt accessionP14618
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CTHBP antibody
- OIP3 antibody
- PKM antibody
see all
Database links
- Entrez Gene: 5315 Human
- Entrez Gene: 18746 Mouse
- Entrez Gene: 25630 Rat
- Omim: 179050 Human
- SwissProt: P14618 Human
- SwissProt: P52480 Mouse
- SwissProt: P11980 Rat
- Unigene: 534770 Human
see all
Anti-PKM2 antibody images:
Western blot - PKM2 antibody (ab38237)
Anti-PKM2 antibody (ab38237) at 1/100 dilution + HeLa cell lysate
Predicted band size : 58 kDa
Observed band size : 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab38237)
ab38237, at 1/50 dilution, staining PKM2 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. ab38237 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Immunocytochemistry/ Immunofluorescence-PKM2 antibody(ab38237)
ICC/IF image of ab38237 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-PKM2 antibody (ab38237)
This product has been referenced in:
- Galino Jet al. Oxidative Damage Compromises Energy Metabolism in the Axonal Degeneration Mouse Model of X-Adrenoleukodystrophy. Antioxid Redox Signal : (2011). WB; Human, Mouse.Read more (PubMed: 21453200) »
- Wittwer JAet al. Enhancing mitochondrial respiration suppresses tumor promoter TPA-induced PKM2 expression and cell transformation in skin epidermal JB6 cells. Cancer Prev Res (Phila) 4:1476-84 (2011). WB; Human.Read more (PubMed: 21673231) »
See all 8 publications for this product
Publishing research using ab38237? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"