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Western blot - PKM2 antibody (ab38237)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab38237)
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Immunocytochemistry/ Immunofluorescence-PKM2 antibody(ab38237)
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Product Name
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PKM2 antibody
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See all PKM2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PKM2
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Immunogen
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KLH conjugated synthetic peptide selected from the C-terminal region of human PKM2.
BLAST 'NFAMNVGKARGFFKK' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Rat, Mk
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate, Human breast carcinoma tissue.
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Cellular localization
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Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform specific and independent of its enzymatic actvity.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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PKM2 (pyruvate kinase, muscle) catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. It has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. Ab38237 recognizes the M1/M2 isoform of pyruvate kinase
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PKM2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PKM2 antibody images, references, products related to ab38237 and other tools.
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PKM2 antibody images:
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 Western blot - PKM2 antibody (ab38237)
PKM2 antibody (ab38237) at 1/100 dilution + HeLa cell lysate
Predicted band size : 58 kDa
Observed band size : 58 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab38237)
ab38237, at 1/50 dilution, staining PKM2 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. ab38237 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
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Immunocytochemistry/ Immunofluorescence-PKM2 antibody(ab38237)
ICC/IF image of ab38237 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for PKM2 antibody (ab38237)
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This product has been used in: (two most recent references)
Rafacho A et al. Glucocorticoids in vivo induce both insulin hypersecretion and enhanced glucose sensitivity of stimulus-secretion coupling in isolated rat islets. Endocrinology 151:85-95 (2010). WB; Rat. PubMed: 19880808
Huang SK et al. LC/MS-based quantitative proteomic analysis of paraffin-embedded archival melanomas reveals potential proteomic biomarkers associated with metastasis. PLoS One 4:e4430 (2009). IHC-P; Human. PubMed: 19221597
See all 4 publication references for this product.
If you publish research using ab38237 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PKM2
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PKM2 antibody - more information
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Customer reviews (feedback) regarding PKM2 antibody |
Customer FAQs regarding PKM2 antibody |
Protocols for PKM2 antibody |
Price and availability of products related to PKM2 antibody |
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PKM2 antibody - related products:
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PKM2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PKM2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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