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Our user would like to know isozyme type of ab55602. Pyruvate Kinase have 4 isozyme as L, R, M1 M2. Which one is isozyme type? |
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ANSWER: |
Thank you for your enquiry. The protein-protein blasting results show that the Pyruvate Kinase is M2 isozyme type. I hope that the information might be helpful. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 58 kDa
PKM2 antibody (ab55602) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Overlay histogram showing HeLa cells stained with ab55602 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55602, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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