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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-PKM2 antibody
See all PKM2 products (8) ...
Description
Mouse monoclonal to PKM2
Tested applications
WB, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-532 of Human PKM2
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Isotype
IgM
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 58 kDa.
Flow Cyt: Use 1µg for 106 cells.
Target
Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities
Belongs to the pyruvate kinase family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated.
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
Target information above from: UniProt accessionP14618
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CTHBP antibody
- OIP3 antibody
- PKM antibody
see all
Database links
- Entrez Gene: 5315 Human
- Omim: 179050 Human
- SwissProt: P14618 Human
- Unigene: 534770 Human
Anti-PKM2 antibody images:
Western blot - PKM2 antibody (ab55602)
Predicted band size : 58 kDa
PKM2 antibody (ab55602) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry - Anti-PKM2 antibody (ab55602)
Overlay histogram showing HeLa cells stained with ab55602 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55602, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-PKM2 antibody (ab55602)
This product has been referenced in:
- Gao Z & Xu CW Glucose metabolism induces mono-ubiquitination of histone H2B in mammalian cells. Biochem Biophys Res Commun 404:428-33 (2011). WB; Human.Read more (PubMed: 21130743) »
See 1 publication for this product
Publishing research using ab55602? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"