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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-PKM2 antibody
See all PKM2 products (8) ...
Description
Goat polyclonal to PKM2
Specificity
The antibody is raised against pyruvate kinase isolated from rabbit muscle. Reactivity with specific PK (PKM1, PKLR) has not been assessed.
Tested applications
ELISA, WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Rabbit, Human
Predicted to work with
all Mammals
Immunogen
Purified pyruvate kinase from Rabbit muscle.
Positive control
Uterus tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab79070)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab79070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/4000 - 1/20000.ELISA: 1/4000 - 1/20000.
WB: 1/500 - 1/2000.Predicted molecular weight: 58 kDa.
IP: Use at an assay dependent dilution.
IHC-P: Use a concentration of 5 µg/ml
Target
Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities
Belongs to the pyruvate kinase family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated.
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
Target information above from: UniProt accessionP14618
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CTHBP antibody
- OIP3 antibody
- PKM antibody
see all
Database links
- Entrez Gene: 5315 Human
- Omim: 179050 Human
- SwissProt: P14618 Human
- SwissProt: P11974 Rabbit
- Unigene: 534770 Human
Anti-PKM2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab79070)
ab79070, at 5µg/ml, staining PKM2 in uterus tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
References for Anti-PKM2 antibody (ab79070)
ab79070 has not yet been referenced specifically in any publications.
Publishing research using ab79070? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"