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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-PKM2 antibody
See all PKM2 products (8) ...
Description
Rabbit polyclonal to PKM2
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Monkey
Immunogen
Synthetic peptide: L IAREAEAA IY HLQLFEE LRR LAPITS DPTE ATAVG AVEASFKCCS GAIIV LTKS GRSAHQ conjugated to KLH, corresponding to amino acids 380-440 of Human PKM2
L IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVG AVEASFKCCS GAIIV LTKSGRSAHQ
Positive control
Tonsil tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab85542)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab85542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 58 kDa.
IHC-P: 1/100
IHC-Fr
IHC-Fr: 1/100IHC-Fr: 1/100
Target
Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities
Belongs to the pyruvate kinase family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated.
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
Target information above from: UniProt accessionP14618
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CTHBP antibody
- OIP3 antibody
- PKM antibody
see all
Database links
- Entrez Gene: 5315 Human
- Entrez Gene: 18746 Mouse
- Entrez Gene: 25630 Rat
- Omim: 179050 Human
- SwissProt: P14618 Human
- SwissProt: P52480 Mouse
- SwissProt: P11980 Rat
- Unigene: 534770 Human
see all
Anti-PKM2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab85542)
ab85542, at 1/100 dilution, staining PKM2 in formalin-fixed paraffin-embedded human tonsil tissue by Immunohistochemistry.
References for Anti-PKM2 antibody (ab85542)
ab85542 has not yet been referenced specifically in any publications.
Publishing research using ab85542? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"