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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab85542)
(enlarge)
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Product Name
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PKM2 antibody
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See all PKM2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PKM2
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Immunogen
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Synthetic peptide: L IAREAEAA IY HLQLFEE LRR LAPITS DPTE ATAVG AVEASFKCCS GAIIV LTKS GRSAHQ conjugated to KLH, corresponding to amino acids 380-440 of Human PKM2
BLAST 'L IAREAEAA IY HLQLFEE LRR LAPITS DPTE ATAVG AVEASFKCCS GAIIV LTKS ' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Mk
(Due to sequence homology)
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Tested applications
(see key)
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IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/100.
IHC-Fr: 1/100.
WB: 1/1000. Predicted molecular weight: 58 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Tonsil tissue.
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Cellular localization
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Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform specific and independent of its enzymatic actvity.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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PKM2 (pyruvate kinase, muscle) catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. It has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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0.090 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PKM2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PKM2 antibody images, references, products related to ab85542 and other tools.
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PKM2 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKM2 antibody (ab85542)
ab85542, at 1/100 dilution, staining PKM2 in formalin-fixed paraffin-embedded human tonsil tissue by Immunohistochemistry.
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Search PubMed (MEDLINE) for references to PKM2
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PKM2 antibody - more information
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Customer reviews (feedback) regarding PKM2 antibody |
Customer FAQs regarding PKM2 antibody |
Protocols for PKM2 antibody |
Price and availability of products related to PKM2 antibody |
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PKM2 antibody - related products:
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PKM2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PKM2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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