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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
PKM2 protein
See all PKM2 products (4) ...
Protein description
Recombinant full length Human PKM2 with an N-terminal hexahistidine tag
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 20mM Tris, pH 8
Concentration
Concentration information loading...
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
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SDS-PAGE - PKM2 protein (ab53384)
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Applications
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Our Abpromise guarantee covers the use of ab53384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities
Belongs to the pyruvate kinase family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated.
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
Target information above from: UniProt accessionP14618
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
PKM2 protein images:
References for PKM2 protein (ab53384)
ab53384 has not yet been referenced specifically in any publications.
Publishing research using ab53384? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"