Anti-PKN2 antibody (ab87812)

Overview

• Product nameAnti-PKN2 antibodySee all PKN2 primary antibodies ...
• Description
  Rabbit polyclonal to PKN2
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to a region between residue 200 and 250 of human PKN2 (NP_006247.1).

• Positive controlWhole cell lysates from HeLa and 293T cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab87812 was affinity purified using an epitope specific to PKN2 immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKC

Applications

Target

• FunctionExhibits a preference for highly basic protein substrates.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 C2 domain.
  Contains 1 protein kinase domain.
  Contains 3 REM (Hr1) repeats.
• DomainThe C1 domain does not bind the diacylglycerol (DAG).
• Post-translational
  modificationsAutophosphorylated.
  Activated by limited proteolysis with trypsin.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q16513 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5586 Human
  • Entrez Gene: 5586 Human
  • Omim: 602549 Human
  • SwissProt: Q16513 Human
  • SwissProt: Q16513 Human
  • Unigene: 440833 Human

• Alternative names
  Cardiolipin activated protein kinase Pak2 antibodyMGC150606 antibodyMGC71074 antibody

  PAK 2 antibodyPAK2 antibodyPKN2 antibodyPKN2_HUMAN antibodyPRK2 antibodyPRKCL2 antibodyPRO2042 antibodyProtein kinase C like 2 antibodyProtein kinase C related kinase 2 antibodyProtein kinase C-like 2 antibodyProtein kinase N2 antibodyProtein-kinase C-related kinase 2 antibodySerine/threonine-protein kinase N2 antibody

  see all

Anti-PKN2 antibody images

• 

  Western blot - PKN2 antibody (ab87812)
  All lanes : Anti-PKN2 antibody (ab87812) at 0.04 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  Lane 4 : 293T whole cell lysate at 50 µg
  
  developed using the ECL technique
  
  Predicted band size : 112 kDa
  Observed band size : 117 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 10 seconds
• 

  Immunoprecipitation - PKN2 antibody (ab87812)
  Detection of PKN2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87812 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
  Detection: Chemiluminescence with an exposure time of 10 seconds.