Overview

  • Product nameAnti-PKN2 antibody
    See all PKN2 primary antibodies
  • Description
    Rabbit polyclonal to PKN2
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between residue 200 and 250 of human PKN2 (NP_006247.1).

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab87812 was affinity purified using an epitope specific to PKN2 immobilized on solid support.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab87812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 112 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionExhibits a preference for highly basic protein substrates.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 1 protein kinase domain.
      Contains 3 REM (Hr1) repeats.
    • DomainThe C1 domain does not bind the diacylglycerol (DAG).
    • Post-translational
      modifications
      Autophosphorylated.
      Activated by limited proteolysis with trypsin.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiolipin activated protein kinase Pak2 antibody
      • MGC150606 antibody
      • MGC71074 antibody
      • PAK 2 antibody
      • PAK2 antibody
      • PKN gamma antibody
      • PKN2 antibody
      • PKN2_HUMAN antibody
      • PRK2 antibody
      • PRKCL2 antibody
      • PRO2042 antibody
      • Protein kinase C like 2 antibody
      • Protein kinase C related kinase 2 antibody
      • Protein kinase C-like 2 antibody
      • Protein kinase N2 antibody
      • Protein-kinase C-related kinase 2 antibody
      • Serine/threonine-protein kinase N2 antibody
      see all

    Anti-PKN2 antibody images

    • All lanes : Anti-PKN2 antibody (ab87812) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      developed using the ECL technique

      Predicted band size : 112 kDa
      Observed band size : 117 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds
    • Detection of PKN2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87812 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-PKN2 antibody (ab87812)

    ab87812 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"