![Western blot - PKR antibody [YE350] (ab32052) image](#ab32052wb.gif)
![Immunohistochemistry (Paraffin-embedded sections) - PKR antibody [YE350] (ab32052) image](#ab32052ihc.jpg)
![Immunocytochemistry/ Immunofluorescence - PKR antibody [YE350] (ab32052) image](#ab32052if.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-PKR antibody [YE350]
See all PKR products (16) ...
Description
Rabbit monoclonal [YE350] to PKR
Specificity
ab32052 recognises Protein kinase R (PKR). It does not cross-react with other GCN2 family members.
Tested applications
WB, IHC-P, ICC/IF, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues following the first double-stranded RNA-binding domain of human PKR.
Positive control
HeLa cell lysate and cells and human stomach carcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
YE350
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.
IP: 1/100.
WB: 1/1000 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
Target information above from: UniProt accessionP19525
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- double stranded RNA activated protein kinase; antibody
- E2AK2_HUMAN antibody
- eIF-2A protein kinase 2 antibody
see all
Database links
- Entrez Gene: 5610 Human
- Omim: 176871 Human
- SwissProt: Q52M43 Human
- SwissProt: P19525 Human
- Unigene: 131431 Human
Anti-PKR antibody [YE350] images:
Western blot - PKR antibody [YE350] (ab32052)
![Western blot - PKR antibody [YE350] (ab32052)](/ps/datasheet/Images/32/ab32052/ab32052wb.gif)
Anti-PKR antibody [YE350] (ab32052) at 1/5000 dilution + HeLa cell lysate
Predicted band size : 62 kDa
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - PKR antibody [YE350] (ab32052)
![Immunohistochemistry (Paraffin-embedded sections) - PKR antibody [YE350] (ab32052)](/ps/datasheet/Images/32/ab32052/ab32052ihc.jpg)
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using ab32052 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - PKR antibody [YE350] (ab32052)
![Immunocytochemistry/ Immunofluorescence - PKR antibody [YE350] (ab32052)](/ps/datasheet/Images/32/ab32052/ab32052if.jpg)
Immunofluorescent staining of HeLa cells using ab32052 at 1/250 dilution.
References for Anti-PKR antibody [YE350] (ab32052)
This product has been referenced in:
- Treppendahl MB et al. Allelic methylation levels of the noncoding VTRNA2-1 located on chromosome 5q31.1 predict outcome in AML. Blood 119:206-16 (2012). WB; Human.Read more (PubMed: 22058117) »
- Teramachi J et al. Double stranded RNA-dependent protein kinase is involved in osteoclast differentiation of RAW264.7 cells in vitro. Exp Cell Res : (2010).Read more (PubMed: 20728438) »
See all 3 publications for this product
Publishing research using ab32052? Please let us know so that we can cite the reference in this datasheet
![PKR antibody [YE350] for WB in Human (32052)](/ps/Reviews/Images/ab13808_82211.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"