Anti-PKR antibody (ab45427)
- Product nameAnti-PKR antibodySee all PKR primary antibodies ...
- DescriptionRabbit polyclonal to PKR
- SpecificityTargets the N terminus of PKR.
- Tested applicationsIHC-P, WB more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 14 amino acids near the amino terminus of human PKR
- Positive control
- Caco 2 whole cell lysate.
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab45427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use a concentration of 5 µg/ml.|
|WB||WB: Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 62 kDa).|
- FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
- Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain.
modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
- double stranded RNA activated protein kinase; antibody
- E2AK2_HUMAN antibody
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- Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
- Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
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- HGNC:9437 antibody
- Interferon induced double stranded RNA activated protein kinase antibody
- Interferon inducible elF2 alpha kinase antibody
- Interferon inducible RNA dependent protein kinase antibody
- Interferon inducible RNA-dependent protein kinase antibody
- Interferon-induced, double-stranded RNA-activated protein kinase antibody
- Interferon-inducible RNA-dependent protein kinase antibody
- MGC126524 antibody
- P1/eIF-2A protein kinase antibody
- P1/eIF2A protein kinase antibody
- p68 kinase antibody
- PKR antibody
- PRKR antibody
- Protein kinase interferon inducible double stranded RNA dependent antibody
- Protein kinase RNA activated antibody
- Protein kinase RNA-activated antibody
- Serine/threonine protein kinase TIK antibody
- Serine/threonine-protein kinase TIK antibody
- Tyrosine protein kinase EIF2AK2 antibody
Anti-PKR antibody images
Lane 1 : Anti-PKR antibody (ab45427) at 1 µg/ml
Lane 2 : Anti-PKR antibody (ab45427) at 2 µg/ml
Lane 1 : Caco 2 whole cell lysate
Lane 2 : Caco 2 whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size : 62 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Additional bands at : 39 kDa. We are unsure as to the identity of these extra bands.
ab45427 at 5µg/ml staining PKR in rat lung tissue by IHC
References for Anti-PKR antibody (ab45427)
ab45427 has not yet been referenced specifically in any publications.