Overview

  • Product nameAnti-PKR antibody
    See all PKR primary antibodies
  • Description
    Rabbit polyclonal to PKR
  • SpecificityTargets the N terminus of PKR.
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 14 amino acids near the amino terminus of human PKR

  • Positive control
    • Caco 2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab45427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 62 kDa).

Target

  • FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
  • Information by UniProt
  • Database links
  • Alternative names
    • double stranded RNA activated protein kinase; antibody
    • E2AK2_HUMAN antibody
    • eIF-2A protein kinase 2 antibody
    • EIF2AK1 antibody
    • EIF2AK2 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
    • eukaryotic translation initiation factor 2-alpha kinase antibody
    • HGNC:9437 antibody
    • Interferon induced double stranded RNA activated protein kinase antibody
    • Interferon inducible elF2 alpha kinase antibody
    • Interferon inducible RNA dependent protein kinase antibody
    • Interferon inducible RNA-dependent protein kinase antibody
    • Interferon-induced, double-stranded RNA-activated protein kinase antibody
    • Interferon-inducible RNA-dependent protein kinase antibody
    • MGC126524 antibody
    • P1/eIF-2A protein kinase antibody
    • P1/eIF2A protein kinase antibody
    • p68 kinase antibody
    • PKR antibody
    • PRKR antibody
    • Protein kinase interferon inducible double stranded RNA dependent antibody
    • Protein kinase RNA activated antibody
    • Protein kinase RNA-activated antibody
    • Serine/threonine protein kinase TIK antibody
    • Serine/threonine-protein kinase TIK antibody
    • Tyrosine protein kinase EIF2AK2 antibody
    see all

Anti-PKR antibody images

  • Lane 1 : Anti-PKR antibody (ab45427) at 1 µg/ml
    Lane 2 : Anti-PKR antibody (ab45427) at 2 µg/ml

    Lane 1 : Caco 2 whole cell lysate
    Lane 2 : Caco 2 whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    anti-rabbit HRP

    Predicted band size : 62 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa. We are unsure as to the identity of these extra bands.
  • ab45427 at 5µg/ml staining PKR in rat lung tissue by IHC

References for Anti-PKR antibody (ab45427)

ab45427 has not yet been referenced specifically in any publications.

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