Anti-PKR antibody (ab45427)

Overview

• Product nameAnti-PKR antibodySee all PKR primary antibodies ...
• Description
  Rabbit polyclonal to PKR
• SpecificityTargets the N terminus of PKR.
• Tested applicationsIHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to 14 amino acids near the amino terminus of human PKR

• Positive controlCaco 2 whole cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Applications

Target

• FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
  Contains 2 DRBM (double-stranded RNA-binding) domains.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.

Target information above from: UniProt accession P19525 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5610 Human
  • Entrez Gene: 5610 Human
  • Entrez Gene: 19106 Mouse
  • Entrez Gene: 54287 Rat
  • Omim: 176871 Human
  • SwissProt: Q52M43 Human
  • SwissProt: P19525 Human
  • SwissProt: P19525 Human

  • SwissProt: Q91YN2 Mouse
  • SwissProt: Q03963 Mouse
  • SwissProt: Q63184 Rat
  • Unigene: 131431 Human
  • Unigene: 378990 Mouse
  • Unigene: 10022 Rat

  see all

• Alternative names
  double stranded RNA activated protein kinase; antibodyE2AK2_HUMAN antibodyeIF-2A protein kinase 2 antibody

  EIF2AK1 antibodyEIF2AK2 antibodyEukaryotic translation initiation factor 2 alpha kinase 2 antibodyEukaryotic translation initiation factor 2-alpha kinase 2 antibodyeukaryotic translation initiation factor 2-alpha kinase antibodyHGNC:9437 antibodyInterferon induced double stranded RNA activated protein kinase antibodyInterferon inducible elF2 alpha kinase antibodyInterferon inducible RNA dependent protein kinase antibodyInterferon inducible RNA-dependent protein kinase antibodyInterferon-induced, double-stranded RNA-activated protein kinase antibodyInterferon-inducible RNA-dependent protein kinase antibodyMGC126524 antibodyP1/eIF-2A protein kinase antibodyP1/eIF2A protein kinase antibodyp68 kinase antibodyPKR antibodyPRKR antibodyProtein kinase interferon inducible double stranded RNA dependent antibodyProtein kinase RNA activated antibodyProtein kinase RNA-activated antibodySerine/threonine protein kinase TIK antibodySerine/threonine-protein kinase TIK antibodyTyrosine protein kinase EIF2AK2 antibody

  see all

Anti-PKR antibody images

• 

  Western blot - PKR antibody (ab45427)
  Lane 1 : Anti-PKR antibody (ab45427) at 1 µg/ml
  Lane 2 : Anti-PKR antibody (ab45427) at 2 µg/ml
  
  Lane 1 : Caco 2 whole cell lysate
  Lane 2 : Caco 2 whole cell lysate
  
  Lysates/proteins at 35 µg per lane.
  
  Secondary
  anti-rabbit HRP
  
  Predicted band size : 62 kDa
  Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 39 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKR antibody (ab45427)

  ab45427 at 5µg/ml staining PKR in rat lung tissue by IHC