Anti-PKR (phospho T258) antibody (ab79310)

Overview

• Product nameAnti-PKR (phospho T258) antibodySee all PKR primary antibodies ...
• Description
  Rabbit polyclonal to PKR (phospho T258)
• Specificityab79310 detects endogenous levels of PKR only when phosphorylated at threonine 258.
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 258 (K-Y-T^P-V-D).

• Positive controlHuman breast carcinoma tissue and Jurkat cell extract (starved for 24 hours).

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab79310 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Applications

Target

• FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
  Contains 2 DRBM (double-stranded RNA-binding) domains.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.

Target information above from: UniProt accession P19525 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5610 Human
  • Entrez Gene: 5610 Human
  • Omim: 176871 Human
  • SwissProt: Q52M43 Human
  • SwissProt: P19525 Human
  • SwissProt: P19525 Human
  • Unigene: 131431 Human

• Alternative names
  Double stranded RNA activated protein kinase; antibodyE2AK2_HUMAN antibodyeIF-2A protein kinase 2 antibody

  EIF2AK1 antibodyEIF2AK2 antibodyEukaryotic translation initiation factor 2 alpha kinase 2 antibodyEukaryotic translation initiation factor 2-alpha kinase 2 antibodyHGNC:9437 antibodyInterferon induced double stranded RNA activated protein kinase antibodyInterferon inducible elF2 alpha kinase antibodyInterferon inducible RNA dependent protein kinase antibodyInterferon-induced, double-stranded RNA-activated protein kinase antibodyInterferon-inducible RNA-dependent protein kinase antibodyMGC126524 antibodyP1/eIF-2A protein kinase antibodyP1/eIF2A protein kinase antibodyp68 kinase antibodyPKR antibodyPRKR antibodyProtein kinase interferon inducible double stranded RNA dependent antibodyProtein kinase RNA activated antibodyProtein kinase RNA-activated antibodySerine/threonine protein kinase TIK antibodyTyrosine protein kinase EIF2AK2 antibody

  see all

Anti-PKR (phospho T258) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKR (phospho T258) antibody (ab79310)
  ab79310, at 1/50 dilution, staining PKR in human breast carcinoma tissue by Immunohistochemistry in the presence (right) or absence (left) of immunizing peptide. Formalin-fixed, paraffin-embedded section.
• 

  Western blot - PKR (phospho T258) antibody (ab79310)
  All lanes : Anti-PKR (phospho T258) antibody (ab79310) at 1/500 dilution
  
  Lane 1 : Jurkat cells starved for 24 hours
  Lane 2 : Jurkat cells starved for 24 hours with Immunizing phosphopeptide at 5 µg
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 62 kDa
  Observed band size : 62 kDa