![Western blot - PKR (phospho T446) antibody [E120] (ab32036) image](#ab32036wb.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKR (phospho T446) antibody [E120] (ab32036) image](#ab32036ihc.jpg)
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Product name
Anti-PKR (phospho T446) antibody [E120]
See all PKR products (16) ...
Description
Rabbit monoclonal [E120] to PKR (phospho T446)
Specificity
The antibody only detects PKR phosphorylated on Threonine 446.
Tested applications
IHC-P, WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Does not react with
Rat
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr446 of human PKR (Interferon-inducible RNA-dependent protein kinase)
Positive control
Hela cell lysate and normal colon tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
E120
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
ICC/IF
ICC/IF: 1/100.ICC/IF: 1/100.
IP
IP: 1/10.IP: 1/10.
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
Target information above from: UniProt accessionP19525
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- double stranded RNA activated protein kinase; antibody
- E2AK2_HUMAN antibody
- eIF-2A protein kinase 2 antibody
see all
Database links
- Entrez Gene: 5610 Human
- Entrez Gene: 19106 Mouse
- Omim: 176871 Human
- SwissProt: Q52M43 Human
- SwissProt: P19525 Human
- SwissProt: Q91YN2 Mouse
- SwissProt: Q03963 Mouse
- Unigene: 131431 Human
see all
Anti-PKR (phospho T446) antibody [E120] images:
Western blot - PKR (phospho T446) antibody [E120] (ab32036)
![Western blot - PKR (phospho T446) antibody [E120] (ab32036)](/ps/datasheet/Images/32/ab32036/ab32036wb.jpg)
All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Hela cell lysate + IFN + CalyculinA
Lane 3 : Hela cell lysate + Pptase
Predicted band size : 62 kDa
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKR (phospho T446) antibody [E120] (ab32036)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKR (phospho T446) antibody [E120] (ab32036)](/ps/datasheet/Images/32/ab32036/ab32036ihc.jpg)
Immunohistochemical analysis of PKR (phospho T446) expression in normal colon tissue, using 1/50 ab32036.
References for Anti-PKR (phospho T446) antibody [E120] (ab32036)
This product has been referenced in:
- Treppendahl MB et al. Allelic methylation levels of the noncoding VTRNA2-1 located on chromosome 5q31.1 predict outcome in AML. Blood 119:206-16 (2012). WB; Human.Read more (PubMed: 22058117) »
- Peidis P et al. Doxorubicin bypasses the cytoprotective effects of eIF2a phosphorylation and promotes PKR-mediated cell death. Cell Death Differ 18:145-54 (2011). WB; Mouse.Read more (PubMed: 20559319) »
See all 9 publications for this product
Publishing research using ab32036? Please let us know so that we can cite the reference in this datasheet
![PKR (phospho T446) antibody [E120] for WB in Human (32036)](/ps/datasheet/images/32/ab32036/PKR-Primary-antibodies-ab32036-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"