Overview

  • Product nameAnti-PKR (phospho T446) antibody
    See all PKR primary antibodies
  • Description
    Rabbit polyclonal to PKR (phospho T446)
  • SpecificityDetects endogenous levels of PKR only when phosphorylated at threonine 446.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesised phosphopeptide derived from PKR around the phosphorylation site of threonine 446 (Human).

  • Positive control
    • K562 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab47377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/10000.
    IHC-P: 1/50 - 1/100.
    WB: Use at an assay dependent dilution. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
    • Information by UniProt
    • Database links
    • Alternative names
      • Double stranded RNA activated protein kinase; antibody
      • E2AK2_HUMAN antibody
      • eIF-2A protein kinase 2 antibody
      • EIF2AK1 antibody
      • EIF2AK2 antibody
      • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
      • HGNC:9437 antibody
      • Interferon induced double stranded RNA activated protein kinase antibody
      • Interferon inducible elF2 alpha kinase antibody
      • Interferon inducible RNA dependent protein kinase antibody
      • Interferon-induced, double-stranded RNA-activated protein kinase antibody
      • Interferon-inducible RNA-dependent protein kinase antibody
      • MGC126524 antibody
      • P1/eIF-2A protein kinase antibody
      • P1/eIF2A protein kinase antibody
      • p68 kinase antibody
      • PKR antibody
      • PRKR antibody
      • Protein kinase interferon inducible double stranded RNA dependent antibody
      • Protein kinase RNA activated antibody
      • Protein kinase RNA-activated antibody
      • Serine/threonine protein kinase TIK antibody
      • Tyrosine protein kinase EIF2AK2 antibody
      see all

    Anti-PKR (phospho T446) antibody images

    • All lanes : Anti-PKR (phospho T446) antibody (ab47377)

      Lane 1 : Extracts from K562 cells treated with Starvation
      Lane 2 : Untreated extracts from K562 cells


      Predicted band size : 62 kDa

    References for Anti-PKR (phospho T446) antibody (ab47377)

    ab47377 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab47377.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"