Anti-PKR (phospho T446) antibody (ab47377)

Overview

• Product nameAnti-PKR (phospho T446) antibodySee all PKR primary antibodies ...
• Description
  Rabbit polyclonal to PKR (phospho T446)
• SpecificityDetects endogenous levels of PKR only when phosphorylated at threonine 446.
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthesised phosphopeptide derived from PKR around the phosphorylation site of threonine 446 (Human).

• Positive controlK562 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Applications

Target

• FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
  Contains 2 DRBM (double-stranded RNA-binding) domains.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.

Target information above from: UniProt accession P19525 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5610 Human
  • Entrez Gene: 5610 Human
  • Entrez Gene: 19106 Mouse
  • Omim: 176871 Human
  • SwissProt: Q52M43 Human
  • SwissProt: P19525 Human
  • SwissProt: P19525 Human
  • SwissProt: Q91YN2 Mouse

  • SwissProt: Q03963 Mouse
  • Unigene: 131431 Human
  • Unigene: 378990 Mouse

  see all

• Alternative names
  Double stranded RNA activated protein kinase; antibodyE2AK2_HUMAN antibodyeIF-2A protein kinase 2 antibody

  EIF2AK1 antibodyEIF2AK2 antibodyEukaryotic translation initiation factor 2 alpha kinase 2 antibodyEukaryotic translation initiation factor 2-alpha kinase 2 antibodyHGNC:9437 antibodyInterferon induced double stranded RNA activated protein kinase antibodyInterferon inducible elF2 alpha kinase antibodyInterferon inducible RNA dependent protein kinase antibodyInterferon-induced, double-stranded RNA-activated protein kinase antibodyInterferon-inducible RNA-dependent protein kinase antibodyMGC126524 antibodyP1/eIF-2A protein kinase antibodyP1/eIF2A protein kinase antibodyp68 kinase antibodyPKR antibodyPRKR antibodyProtein kinase interferon inducible double stranded RNA dependent antibodyProtein kinase RNA activated antibodyProtein kinase RNA-activated antibodySerine/threonine protein kinase TIK antibodyTyrosine protein kinase EIF2AK2 antibody

  see all

Anti-PKR (phospho T446) antibody images

• 

  Western blot - PKR (phospho T446) antibody (ab47377)
  All lanes : Anti-PKR (phospho T446) antibody (ab47377)
  
  Lane 1 : Extracts from K562 cells treated with Starvation
  Lane 2 : Untreated extracts from K562 cells
  
  
  Predicted band size : 62 kDa