Anti-PLA1A/NMD antibody (ab233145)
Key features and details
- Rabbit polyclonal to PLA1A/NMD
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-PLA1A/NMD antibody -
Description
Rabbit polyclonal to PLA1A/NMD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment (His-tag) corresponding to Human PLA1A/NMD aa 300 to the C-terminus. (Expressed in E.coli).
Database link: Q53H76 -
Positive control
- WB: HEK-293T cell lysate; Pig liver lysate; Recombinant human PLA1A/NMD protein. IHC-P: Human lung and kidney tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab233145 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production. -
Tissue specificity
Widely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 51365 Human
- Entrez Gene: 100171726 Orangutan
- Entrez Gene: 100154368 Pig
- Omim: 607460 Human
- SwissProt: Q53H76 Human
- SwissProt: Q5RBQ5 Orangutan
- Unigene: 437451 Human
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Alternative names
- AA986889 antibody
- NMD antibody
- OTTHUMP00000215248 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human lung tissue stained for PLA1A/NMD using ab233145 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human kidney tissue stained for PLA1A/NMD using ab233145 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-PLA1A/NMD antibody (ab233145) at 1.5 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig liver lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 50 kDa -
Anti-PLA1A/NMD antibody (ab233145) at 2 µg/ml + Recombinant human PLA1A/NMD protein
Predicted band size: 50 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233145 has not yet been referenced specifically in any publications.