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Overview

  • Product nameAnti-PLA2G12B antibody
  • Description
    Rabbit polyclonal to PLA2G12B
  • Tested applicationsICC/IF, WB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the N terminal residues of human PLA2G12B (NP_115951)

  • Positive controlMouse liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.
WB WB: 1/200 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA ELISA: Use at an assay dependent dilution.

Target

  • RelevancePhospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]).
  • Cellular localizationSecreted
  • Database links
    • Alternative names
        2010002E04Rik antibodyFKSG71 antibodyGroup XIIB phospholipase A2 antibody
        Group XIII secreted phospholipase A2 antibodyGXIIB antibodyGXIIIsPLA2 antibodyMGC138151 antibodyPhospholipase A2, group XIIB antibodyPLA2G13 antibody
      see all

    Anti-PLA2G12B antibody images

    • Western blot - PLA2G12B antibody (ab85069)
      Western blot - PLA2G12B antibody (ab85069)
      Anti-PLA2G12B antibody (ab85069) at 1/250 dilution + mouse liver lysates

      Predicted band size : 22 kDa
      Observed band size : 22 kDa
    • Immunocytochemistry/ Immunofluorescence-PLA2G12B antibody(ab85069)
      Immunocytochemistry/ Immunofluorescence-PLA2G12B antibody(ab85069)
      ICC/IF image of ab85069 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85069, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-PLA2G12B antibody (ab85069)

    ab85069 has not yet been referenced specifically in any publications.

    Publishing research using ab85069 ? Please let us know so that we can cite the reference in this datasheet

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    How was the liver lysate prepared for the western blot validation?

    Thank you for contacting us. The liver lysate was not prepared in any special way. It was a standard lysis of fresh mouse liver in RIPA buffer with protease inhibitors. Please do not hesitate to contact us if you need any more advice or information.  

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"