The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/250.
Tissue specificityExpressed at high levels in plasmacytoid dendritic cells. High expression in spleen, lymph nodes, peripheral blood leukocytes, and bone marrow, with lower expression in thymus, appendix, and fetal liver.
Sequence similaritiesBelongs to the cornifelin family.
ab122652 at 1/200 dilution staining PLAC8 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Western blot - Anti-PLAC8 antibody (ab122652)
All lanes : Anti-PLAC8 antibody (ab122652) at 1/250 dilution
Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma lysate Lane 4 : Human liver tissue lysate Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique
References for Anti-PLAC8 antibody (ab122652)
This product has been referenced in:
Blue EK et al. Epigenetic Regulation of Placenta-Specific 8 Contributes to Altered Function of Endothelial Colony-Forming Cells Exposed to Intrauterine Gestational Diabetes Mellitus. Diabetes64:2664-75 (2015).
Read more (PubMed: 25720387) »