Anti-PLCL2 antibody - C-terminal (ab199852)
Key features and details
- Rabbit polyclonal to PLCL2 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLCL2 antibody - C-terminal -
Description
Rabbit polyclonal to PLCL2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human PLCL2 aa 1050 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_001137854.1).
Database link: Q9UPR0 -
Positive control
- HeLa, 293T, and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 126 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 126 kDa. |
Target
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Function
May play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain. -
Cellular localization
Cytoplasm. Predominantly localized to perinuclear areas in both myoblast and myotube C2C12 cells. - Information by UniProt
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Database links
- Entrez Gene: 470763 Chimpanzee
- Entrez Gene: 510408 Cow
- Entrez Gene: 485652 Dog
- Entrez Gene: 101137070 Gorilla
- Entrez Gene: 100063395 Horse
- Entrez Gene: 23228 Human
- Entrez Gene: 100452541 Orangutan
- Entrez Gene: 695590 Rhesus monkey
see all -
Alternative names
- FLJ13484 antibody
- Inactive phospholipase C like protein 2 antibody
- Inactive phospholipase C-like protein 2 antibody
see all
Images
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All lanes : Anti-PLCL2 antibody - C-terminal (ab199852) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 126 kDa
Exposure time: 3 minutesLysates prepared wtih NETN lysis buffer.
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Detection of PLCL2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab199852 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab199852 has not yet been referenced specifically in any publications.