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Overview

  • Product namePLGF protein (Human)See all PLGF proteins and peptides ...
  • Protein descriptionRecombinant full length Human PLGF (amino acids 19-149) expressed in modified human 293 cells (Swiss-Prot P49763-2).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: LPAVPPQQWALSAGNGSSEVEVVPFQEVW GRSYCRALERLVDVVSEYP SEVEHMFSPSCVSLLRCTG CCGDENLHCVPVETANVTMQLLKIRSGDR PSYVELTFS QHVRCECRPLREKMKPERCGDAVPRR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab84148 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab84148 migrates as two broad bands between 17 and 30 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 14.7kDa. ab84148 consists of 0-38% carbohydrate by weight.

    pI: ab84148 separates into a number of glycoforms with varying pI values on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 5.94.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        PGFPGFLPIGF
        Placenta growth factorPlacental growth factorPLGFPLGF_HUMANPlGF2
      see all
  • FunctionGrowth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
  • Tissue specificityWhile the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • DomainIsoform PlGF-2 contains a basic insert which acts as a cell retention signal.
  • Post-translational
    modifications    N-glycosylated.
  • Cellular localizationSecreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.

    • Target information above from: UniProt accession P49763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PLGF protein (Human) images

    • Functional Studies - PIGF protein (ab84148)
      Functional Studies - PIGF protein (ab84148)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - PIGF protein (ab84148)
      SDS-PAGE - PIGF protein (ab84148)
      1D SDS-PAGE of ab84148 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab84148
      Lane 2: ab84148 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab84148 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
      10µg protein loaded per lane; Deep Purple™ stained.

      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.

    References for PLGF protein (Human) (ab84148)

    ab84148 has not yet been referenced specifically in any publications.

    Publishing research using ab84148 ? Please let us know so that we can cite the reference in this datasheet

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