PLGF protein (Human) (ab84148)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab84148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Molecular Mass: ab84148 migrates as two broad bands between 17 and 30 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 14.7kDa. ab84148 consists of 0-38% carbohydrate by weight.
pI: ab84148 separates into a number of glycoforms with varying pI values on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 5.94.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Placenta growth factorPlacental growth factorPLGFPLGF_HUMANPlGF2
PLGF protein (Human) images
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab84148 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab84148
Lane 2: ab84148 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab84148 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10µg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
References for PLGF protein (Human) (ab84148)
ab84148 has not yet been referenced specifically in any publications.