Anti-PLK1 antibody [35-206] (ab17056)

Overview

• Product nameAnti-PLK1 antibody [35-206]See all PLK1 primary antibodies ...
• Description
  Mouse monoclonal [35-206] to PLK1
• Tested applicationsFlow Cyt, ICC/IF, IHC-P, IP, WB, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  His-PLK1 full length purified from Sf9 cells.

• Epitopeaa330-370.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number35-206
• IsotypeIgG2b
• Research Areas
  • Cancer
  • Cell cycle
  • Cell division

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Other

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Other

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Spindle

Applications

Target

• FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
• Tissue specificityPlacenta and colon.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
  Contains 2 POLO box domains.
  Contains 1 protein kinase domain.
• Developmental stageAccumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
• Post-translational
  modificationsCatalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
  Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
  Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
• Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.

Target information above from: UniProt accession P53350 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5347 Human
  • Entrez Gene: 18817 Mouse
  • Entrez Gene: 25515 Rat
  • Omim: 602098 Human
  • SwissProt: P53350 Human
  • SwissProt: Q07832 Mouse
  • SwissProt: Q62673 Rat
  • Unigene: 592049 Human

  • Unigene: 16525 Mouse
  • Unigene: 11034 Rat

  see all

• Alternative names
  Cell cycle regulated protein kinase antibodyPLK 1 antibodyPLK antibody

  PLK-1 antibodyplk1 antibodyPLK1_HUMAN antibodypolio like kinase antibodyPolo like kinase 1 antibodyPolo-like kinase 1 antibodySerine/threonine protein kinase 13 antibodySerine/threonine protein kinase PLK1 antibodySerine/threonine-protein kinase 13 antibodySerine/threonine-protein kinase antibodySerine/threonine-protein kinase PLK1 antibodySTPK 13 antibodySTPK13 antibody

  see all

Anti-PLK1 antibody [35-206] images

• 

  Western blot - Plk1 antibody [35-206] (ab17056)
  All lanes : Anti-PLK1 antibody [35-206] (ab17056)
  
  Lane 1 : Recombinant PLK1
  Lane 2 : U2OS lysate
  Lane 3 : HeLaS3
  
  
  Performed under reducing conditions.
  
  Predicted band size : 67 kDa
  Observed band size : 66 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunofluorescence - Plk1 antibody [35-206] (ab17056)

  Immunofluoresence using ab17056 and either U2OS, HeLaS3 or NIH 3T3 cells.
  
  Plk1 can be seen localising with MKLP in the U2OS cell and with Cdc20 in the HeLaS3 and NIH 3T3 cells shown.

• 

  Western blot - Plk1 antibody [35-206] (ab17056)
  Anti-PLK1 antibody [35-206] (ab17056) at 1 µg/ml + A431 cell lysate at 20 µg
  
  Secondary
  Rabbit-anti mouse Alexa fluor(680) at 1/10000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 67 kDa
  Observed band size : 66 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Plk1 antibody [35-206] (ab17056)This image is courtesy of an Abreview submitted by Melanie Adler
  ab17056 at 1/100 staining human kidney tubular epithelial cells by ICC/IF. The cells were formaldehyde fixed, permeabilized with Triton X-100 and blocked with goat serum before incuabtion with the antibody. A goat anti-mouse FITC antibody was used as the secondary.

  See Abreview

• 

  Flow Cytometry-Anti-PLK1 antibody [35-206](ab17056)
  Overlay histogram showing HCT116 cells stained with ab17056 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17056, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.