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His-PLK1 full length purified from Sf9 cells.
Our Abpromise guarantee covers the use of ab17056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 67 kDa). This clone is superior to clone 36-298 (ab17057) in Western blotting and IP, but suffers from high background in IF and ICC.|
|ICC||Use at an assay dependent concentration.|
Immunofluoresence using ab17056 and either U2OS, HeLaS3 or NIH 3T3 cells.
Plk1 can be seen localising with MKLP in the U2OS cell and with Cdc20 in the HeLaS3 and NIH 3T3 cells shown.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"